文献类型：期刊文献

英文题名：Blood-brain barrier permeability to enrofloxacin in Pengze crucian carp (Carassius auratus var. Pengze)

作者：Zhang, Fan[1];Wang, Runping[1];Huang, Jianzhen[1];Zhang, Haixin[2];Wang, Long[3];Zhong, Zhiwei[1];Ruan, Jiming[1];Liu, Huazhong[4]

机构：[1]Jiangxi Agr Univ, Dept Aquaculture, Coll Anim Sci & Technol, Nanchang 330045, Jiangxi, Peoples R China;[2]Jiangxi Fisheries Res Inst, Nanchang, Jiangxi, Peoples R China;[3]Bur Agr & Rural Affairs Pengze Cty, Pengze, Peoples R China;[4]Guangdong Ocean Univ, Coll Chem & Environm Sci, Zhanjiang 524088, Peoples R China

年份：2022

卷号：8

期号：6

起止页码：2404

外文期刊名：VETERINARY MEDICINE AND SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000847127900001)、、Scopus(收录号：2-s2.0-85136868072)、WOS

基金：National Natural Science Foundation of China, Grant/Award Number: 31860735; Integration and Demonstration of Rice and Fishery Green Ecological Comprehensive Planting and Breeding Technology, Grant/Award Number: 20192ACB60009; Key Project of Science and Technology Plan of Jiangxi Department of Education, Grant/Award Number: GJJ190169; Key R&D Project of Jiangxi Province, Grant/Award Number: 20171BBF60056

语种：英文

外文关键词：blood-brain barrier; drug residues; enrofloxacin; Pengze crucian carp

外文摘要：Background: Enrofloxacin (ENR) is a kind of quinolone antibiotic that is most widely used antimicrobials in veterinary practice, and possesses both a broad spectrum antimicrobial activity against a range of bacteria and adverse effects towards plants and animals. Objectives: This study was conducted to explore the permeability of blood-brain barrier (BBB) to ENR and brain injury based on crucian carp orally administrated with high dose of ENR. Methods: Juvenile Pengze crucian carp were treated with half lethal dose (LD50) or safe dose (SD50) of ENR. BBB permeability was determined by evaluating ENR contents detected by H PLC and evens blue contents estimated by confocal laser scanning microscope. Brain damage was evaluated by measuring protein and m RNA contents of related molecules with western blotting and qPCR. Results: Data indicated that ENR destroyed BBB structure of crucian carp and enhanced permeability of the biological barrier, resulting in more ENR crossed BBB and induced brain damage of crucian carp. Conclusions: This data indicated that ENR can induce brain damage of crucian carp through destroying BBB structure and enhancing permeability.