文献类型：期刊文献

英文题名：Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

作者：Niu, Bowen[1];Li, Bo[1];Wu, Chongyang[1];Wu, Jiang[2];Yan, Yuan[1];Shang, Rui[1];Bai, Chunling[3];Li, Guangpeng[3];Hua, Jinlian[1]

机构：[1]Northwest A&F Univ, Coll Vet Med, Shaanxi Stem Cell Engn & Technol Res Ctr, Yangling 712100, Shaanxi, Peoples R China;[2]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Peoples R China;[3]Inner Mongolia Univ, Minist Educ, Key Lab Mammalian Reprod Biol & Biotechnol, Hohhot 010021, Peoples R China

年份：2016

卷号：7

期号：47

起止页码：77532

外文期刊名：ONCOTARGET

收录：SCI-EXPANDED(收录号：WOS:000389633400097)、、Scopus(收录号：2-s2.0-84998773696)、WOS

基金：This work was supported by grants from the Program of National Natural Science Foundation of China (31272518, 31572399), the China National Basic Research Program (JFYS 2016YFA0100203), the National Major Project for Production of Transgenic Breeding (2014ZX08007-002), National High Technology Research and Development Program of China (SS2014AA021605), the Key Project of the Chinese Ministry of Science and Technology (2013CB967401), and the Program of the Shaanxi Province (2015NY157).

语种：英文

外文关键词：melatonin; spermatogonia stem cells (SSCs); GDNF; Sertoli cells; proliferation

外文摘要：Melatonin has been reported to be an important endogenous hormone for regulating neurogenesis, immunityand the biological clock. Recently, the effects of melatonin on neural stem cells (NSCs), mesenchymal stem cells (MSCs), and induced pluripotent stem cells (iPSCs) have been reported; however, the effects of melatonin on spermatogonia stem cells (SSCs) are not clear. Here, 1 mu M and 1nM melatonin was added to medium when goat SSCs were cultured in vitro, the results showed that melatonin could increase the formation and size of SSC colonies. Real-time quantitative PCR (QRT-PCR) and western blot analysis showed that the expression levels of SSC proliferation and self-renewal markers were up-regulated. Meanwhile, QRT-PCR results showed that melatonin inhibit the mRNA expression level of SSC differentiation markers. ELISA analysis showed an obvious increase in the concentration of GDNF (a niche factor secreted by Sertoli cells) in the medium when treated with melatonin. Meanwhile, the phosphorylation level of AKT, a downstream of GDNF-GFRa1-RET pathway was activated. In conclusion, melatonin promotes goat SSC proliferation by stimulating GDNF production in Sertoli cells.