文献类型：期刊文献

英文题名：CD49f promotes proliferation of male dairy goat germline stem cells

作者：Zheng, Liming[1];Zhu, Haijing[1,2];Mu, Hailong[1];Wu, Jiang[1,3];Song, Wencong[1];Zhai, Yuanxin[1];Peng, Sha[1];Li, Guangpeng[4];Hua, Jinlian[1]

机构：[1]Northwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China;[2]Yulin Univ, Coll Life Sci, Yulin 719000, Shaanxi, Peoples R China;[3]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Guangdong, Peoples R China;[4]Inner Mongolia Univ, Key Lab Mammalian Reprod Biol & Biotechnol, Minist Educ, Hohhot 010021, Peoples R China

年份：2016

卷号：49

期号：1

起止页码：27

外文期刊名：CELL PROLIFERATION

收录：SCI-EXPANDED(收录号：WOS:000370155400003)、、Scopus(收录号：2-s2.0-84958936621)、WOS

基金：The authors appreciate Professor Kang Kyung-Sun's supplying the pCMV6-CD49f plasmid. This work was supported by grants from the Program of National Natural Science Foundation of China (31272518, 31572399), the National Major Project for Production of Transgenic Breeding (2014ZX08007-002), National High Technology Research and Development Program of China (SS2014AA021605), the Key Project of Chinese Ministry of Science and Technology (2013CB967401), Doctoral Fund of Ministry of Education of China (RFDP, 20120204110030).

语种：英文

外文摘要：ObjectivesCD49f enhances multipotency and maintains stemness in embryonic stem cells (ESCs), however, whether it would be effective in mGSCs has remained unclear. Moreover, better standards for mGSC enrichment and purification are necessary. The present study was conducted to determine roles of CD49f in mGSC enrichment and regulation. Materials and methodsCD49f expression patterns were investigated in dairy goats. CD49f positive cells were purified and enriched using magnetic-activated cell sorting (MACS), and characteristics of the cultured cells were assayed using alkaline phosphatase (AP) analysis, quantitative real-time PCR (QRT-PCR) and immunofluorescence analysis. Furthermore, the exogenous CD49f gene was transfected into mGSCs and its effects were analysed. ResultsCD49f was found to be conserved in both mRNA and amino acid sequences and that it was an efficient marker for dairy goat mGSC identification, enrichment and purification. CD49f positive cells expressed higher levels of mGSC-specific markers, and proliferated faster than CD49f negative cells. Overexpression CD49f promoted proliferation of dairy goat mGSCs, and Oct4 expression was upregulated; histone H3-lysine 9 dimethylation (H3K9me2) was reduced. ConclusionsTaken together, our data suggest that CD49f plays novel and dynamic roles in regulating maintenance of pluripotency in mGSCs via Oct4 crosstalk and histone methylation dynamics,which may provide new solutions for mGSCs stability in vitro.