文献类型：期刊文献

中文题名：Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis

英文题名：Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis

作者：WANG Hui[1];YANG Hongshuai[2];LIU Jiahui[2];LI Yanhong[2];LIU Zhigang[2]

机构：[1]School of Life Sciences, Huaiyin Normal University;[2]Fisheries College, Guangdong Ocean University

年份：2016

卷号：35

期号：4

起止页码：51

中文期刊名：Acta Oceanologica Sinica

外文期刊名：海洋学报（英文版）

收录：SCI-EXPANDED(收录号：WOS:000374250700007)、CSTPCD、、CSCD2015_2016、Scopus(收录号：2-s2.0-84963772556)、WOS、CSCD

基金：Foundation item: The Guangdong Province Education Department under contract No. GCZX-A0909; the Guangdong Province Ocean and Fisheries Science & Technology Extension Project under contract No. 20120980; the Guangdong Province Industry-University-Science Partnership Project under contract No. 20110908; the Science & Technology Project of Huaiyin Normal University under contract No. WH0031.

语种：英文

中文关键词：Crassostrea ariakensis superoxide dismutase temperature copper ion concentration combined effect

外文关键词：Crassostrea ariakensis superoxide dismutase temperature copper ion concentration combined effect

中文摘要：Superoxide dismutase（SOD） is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainly two specific forms, Cu/Zn-SOD（SOD1） and Mn-SOD（SOD2）, in eukaryotes. SODs are known to be concurrently modulated by a variety of environmental stressors. By using central composite experimental design and response surface method, the joint effects of water temperature（18–34°C） and copper ion concentration（0.1–1.5 mg/L） on the total SOD activity in the digestive gland of Crassostrea ariakensis were studied. The results showed that the linear effect of temperature was highly significant（P〈0.01）, the quadratic effect of temperature was significant（P〈0.05）; the linear effect of copper ion concentration was not significant（P〉0.05）, while the quadratic effect of copper ion concentration was highly significant（P〈0.01）; the interactive effect of temperature and copper ion concentration was not significant（P〉0.05）; the effect of temperature was greater than that of copper ion concentration. The model equation of digestive gland SOD enzyme activity towards the two factors of interest was established, with R2 and predictive R2 as high as 0.961 6 and 0.820 7, respectively, suggesting that the goodness-offit to experimental data be very satisfactory, and could be applied to prediction of digestive gland SOD activity in C. ariakensis under the conditions of the experiment. Our results would be conducive to addressing the health of aquatic animals and/or to detecting environmental problems by taking SOD as a potential bioindicator.

外文摘要：Superoxide dismutase（SOD） is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainly two specific forms, Cu/Zn-SOD（SOD1） and Mn-SOD（SOD2）, in eukaryotes. SODs are known to be concurrently modulated by a variety of environmental stressors. By using central composite experimental design and response surface method, the joint effects of water temperature（18–34°C） and copper ion concentration（0.1–1.5 mg/L） on the total SOD activity in the digestive gland of Crassostrea ariakensis were studied. The results showed that the linear effect of temperature was highly significant（P〈0.01）, the quadratic effect of temperature was significant（P〈0.05）; the linear effect of copper ion concentration was not significant（P〉0.05）, while the quadratic effect of copper ion concentration was highly significant（P〈0.01）; the interactive effect of temperature and copper ion concentration was not significant（P〉0.05）; the effect of temperature was greater than that of copper ion concentration. The model equation of digestive gland SOD enzyme activity towards the two factors of interest was established, with R2 and predictive R2 as high as 0.961 6 and 0.820 7, respectively, suggesting that the goodness-offit to experimental data be very satisfactory, and could be applied to prediction of digestive gland SOD activity in C. ariakensis under the conditions of the experiment. Our results would be conducive to addressing the health of aquatic animals and/or to detecting environmental problems by taking SOD as a potential bioindicator.