文献类型：期刊文献

英文题名：Regulation by walnut protein hydrolysate on the components and structural degradation of photoaged skin in SD rats

作者：Xu, Defeng[1];Li, Dan[2];Zhao, Zijian[1];Wu, Jiaxin[1];Zhao, Mouming[3]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Guangdong Prov Engn Technol Res Ctr Marine Food, Coll Food Sci & Technol,Key Lab Adv Proc Aquat Pr, Zhanjiang 524088, Peoples R China;[2]Huaibei Normal Univ, Sch Chem & Mat Sci, Huaibei 235000, Peoples R China;[3]South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China

年份：2019

卷号：10

期号：10

起止页码：6792

外文期刊名：FOOD & FUNCTION

收录：SCI-EXPANDED(收录号：WOS:000490983800047)、、EI(收录号：20194307577800)、Scopus(收录号：2-s2.0-85073309593)、WOS

基金：The authors gratefully thank the National Natural Science Foundation (No. 31201309 and 31772048), the State Key Research and Development Plan (No. 2017YFD0400200), the Fundamental Research Funds for the Central Universities (No. 20152M062) and the National College Students' Innovative Entrepreneurial Training Plan (No. 20161056601) for the financial support.

语种：英文

外文关键词：Hydroxyproline - Biochemistry - Glycoproteins - Hyaluronic acid - Hydrolysis - Ultraviolet radiation - Collagen - Rats - Dietary supplements

外文摘要：Skin photoaging induced by consecutive exposure of skin to ultraviolet radiation is primarily responsible for skin aging and preparation of food-derived ingredients with anti-aging functions has been the hot topic worldwide. Dietary consumption of food supplements has been found to modulate skin functions and can be useful in the prevention of skin aging. To evaluate the effect of walnut protein hydrolysate (WPH) on photoaged skin, Sprague-Dawley rats (SD rats) were orally administered with WPH and then were regularly exposed to ultraviolet radiation (UV-R). After a consecutive UV-R for 18 weeks, the delaying efficiency of WPH against elasticity degradation was examined and the mechanical mechanism was explored subsequently. The contents of hydroxyproline (Hyp) and hyaluronic acid (HA) in the extracellular matrix (ECM) were measured by biochemical reactions and color rendering procedures; the levels of types I and III collagen (Col I and III) and the activity of matrix metalloproteinase-1 (MMP-1) were detected by enzyme-linked immunosorbent assay (ELISA); the protein levels of elastin and fibrillin-1 were examined by western blotting. Moreover, the histological change in the skin structure was illustrated by hematoxylin & eosin (HE) and Masson staining. The results revealed that WPH evidently enhanced the elasticity of photoaged skin and stimulated the biosynthesis of ECM components Col I, Hyp and HA in the dermal layer; meanwhile WPH inhibited the MMP-1 activity, alleviated epidermal hyperplasia, and repaired the damaged skin mechanical structure in a dose-dependent manner. In particular, in comparison with the UV-R group, the WPH group in which WPH was administered at a high-dose level showed significantly improved skin appearance, ECM components and structure (P < 0.05). Taken together, the elasticity improvement caused by WPH against the skin photoaging process can be attributed to the regulation of the metabolism of the components and repair of the damaged mechanical structure of the ECM. This research proved the potential of WPH as a functional ingredient for the development of anti-photoaging foods.