文献类型：期刊文献

英文题名：Construction of a Vibrio alginolyticus hopPmaJ (hop) mutant and evaluation of its potential as a live attenuated vaccine in orange-spotted grouper (Epinephelus coioides)

作者：Pang, Huanying[1,2,3];Qiu, Mingsheng[1];Zhao, Jingmin[1];Hoare, Rowena[4];Monaghan, Sean J.[4];Song, Dawei[1,2,3];Chang, Yunsheng[1,2,3];Jian, Jichang[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fishery, 40 East Jiefang Rd, Zhanjiang 524025, Guangdong, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China;[3]Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524025, Peoples R China;[4]Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland

年份：2018

卷号：76

起止页码：93

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000430888400011)、、Scopus(收录号：2-s2.0-85042701353)、WOS

基金：This work was supported by the National Natural Science Foundation of China (No. 31402344), Natural Science Foundation of Guangdong Province (No. 2017A030313174), Outstanding Young Backbone Teacher Cultivation Program of Guangdong Ocean University (No. HDYQ2015005), Project of Enhancing School with innovation of Guangdong Ocean University (No. GDOU2015050216); Basic research free exploration project of Shenzhen science and technology innovation Committee.

语种：英文

外文关键词：Vibrio alginolyticus; T3SS; hopPmaJ; Live attenuated vaccine; Epinephelus coioides

外文摘要：Vibrio alginolyticus, a bacterial pathogen in fish and humans, expresses a type III secretion system (T3SS) that is critical for pathogen virulence and disease development. However, little is known about the associated effectors (T3SEs) and their physiological role. In this study, the T3SE gene hopPmaJ (hop) was cloned from V. alginolyticus wild-type strain HY9901 and the mutant strain HY9901 Delta hop was constructed by the in-frame deletion method. The results showed that the deduced amino acid sequence of V. alginolyticus HopPmaJ shared 78-98% homology with other Vibrio spp. In addition, the HY9901 Delta hop mutant showed an attenuated swarming phenotype and a 2600-fold decrease in the virulence to grouper. However, the HY9901 Delta hop mutant showed no difference in morphology, growth, biofilm formation and ECPase activity. Finally, grouper vaccinated via intraperitoneal (IP) injection with HY9901 Delta hop induced a high antibody titer with a relative percent survival (RPS) value of 84% after challenging with the wild-type HY9901. Real-time PCR assays showed that vaccination with HY9901 Delta hop enhanced the expression of immune-related genes, including MHC-I alpha, MHC-II alpha, IgM, and IL-1 beta after vaccination, indicating that it is able to induce humoral and cell-mediated immune response in grouper. These results demonstrate that the HY9901 Delta hop mutant could be used as an effective live vaccine to combat V. alginolyticus in grouper.