文献类型：期刊文献

英文题名：cDNA CLONING AND EXPRESSION ANALYSIS OF CHAPERONIN CONTAINING T-COMPLEX POLYPEPTIDE 1-ALPHA IN THE PEARL OYSTER PINCTADA MAXIMA EXPOSED TO COLD STRESS

作者：Tang, Xiaochen[1];Mao, Aitao[1];Ding, Yu[1];Liu, Zhigang[1];Liu, Huazhong[2]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, 1 Haida Rd, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Coll Chem & Environm Sci, 1 Haida Rd, Zhanjiang 524088, Peoples R China

年份：2021

卷号：40

期号：3

起止页码：505

外文期刊名：JOURNAL OF SHELLFISH RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000741934800007)、、Scopus(收录号：2-s2.0-85122670781)、WOS

基金：This work was supported by the Marine Fishery Science and Technology Promotion Special Project of Guangdong Province (A201008A01, A201201A06, and A201208A07), The Science and Technology Projects of Guangdong Province (2010B020308008), Improvement Projects for PhD of Guangdong Ocean University (0812269), and Science and Technology Projects of Guangdong Ocean University (Y13411).

语种：英文

外文关键词：Pinctada maxima; CCT-alpha; cDNA; cold stress

外文摘要：The complete cDNA sequence of chaperonin-containing T-complex polypeptide 1-alpha (CCT-alpha) gene of the pearl oyster Pinctada maxima was determined using rapid amplification of cDNA ends technique and characterized with bio-informatic analysis. Its expression profile in different organs and under cold stress was characterized using real-time quantitative polymerase chain reaction. Results revealed that the complete cDNA sequence of P. maxima CCT-alpha has 1,803 base pairs (bp), including a 1,608-bp open reading framework that codes 535 amino acids. The protein has three conservative functional domains: equatorial, apical, and intermediate domains. The equatorial domain is 20 amino acids shorter than that of other species, and contains the three signature sequences of CCT family protein. The CCT-alpha of P. maxima has nine conservative ATP-binding sites and six protein-binding sites, and has high homology with Aplysia californica (80.7%), Delia antiqua (77.4%), Danio rerio (77.1%), Xenopus laevis (76.7%), Gallus gallus (76.4%), and Homo sapiens (75.4%). The CCT-alpha shows tissue-specific expression profile in P. maxima, with expression levels higher in gill, mantle, and adductor muscle than in hepatopancreas and heart. Cold stress markedly increased mRNA contents of CCT-alpha in the organs of P. maxima, but different organs showed distinct cold stress response pattern. Chaperonin-containing T-complex polypeptide 1-alpha transcription in adductor muscle, hepatopancreas, and heart increased at 2 h, and reached peak values at 6 h, maintained high levels at 12 h, and significantly decreased at 24 h. The product in gill and mantle decreased at 2 h, began to increase at 6 h, and showed a large increase at 12 h, but reduced to below the initial level at 24 h.