文献类型：期刊文献

英文题名：Tissue inhibitor of metalloproteinase gene from pearl oyster Pinctada martensii participates in nacre formation

作者：Yan, Fang[1];Jiao, Yu[1];Deng, Yuewen[1];Du, Xiaodong[1];Huang, Ronglian[1];Wang, Qingheng[1];Chen, Weiyao[1]

机构：[1]Guangdong Ocean Univ, Fishery Coll, Zhanjiang City 524025, Guangdong, Peoples R China

年份：2014

卷号：450

期号：1

起止页码：300

外文期刊名：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

收录：SCI-EXPANDED(收录号：WOS:000343641000049)、、Scopus(收录号：2-s2.0-84904725160)、WOS

基金：The studies were financially supported by grants of the National Natural Science Foundation of China (31272635, 31372526 and 41206141), Guangdong Province breeding project fund (2012L YM_0074), Guangdong Natural Science Foundation (S20120 40008042).

语种：英文

外文关键词：Tissue inhibitors of metalloproteinases (TIMPs); Pinctada martensii; Nacre formation

外文摘要：Tissue inhibitors of metalloproteinases (TIMPs) are nature inhibitors of matrix metalloproteinases and play a vital role in the regulation of extracellular matrix turnover, tissue remodeling and bone formation. In this study, the molecular characterization of TIMP and its potential function in nacre formation was described in pearl oyster Pinctada martensii. The cDNA of TIMP gene in P. martensii (Pm-TIMP) was 901 bp long, containing a 5' untranslated region (UTR) of 51 bp, a 3' UTR of 169 bp, and an open reading fragment (ORF) of 681 bp encoding 226 amino acids with an estimated molecular mass of 23.37 kDa and a theoretical isoelectric point of 5.42; The predicted amino acid sequence had a signal peptide, 13 cysteine residues, a N-terminal domain and a C-terminal domain, similar to that from other species. Amino acid multiple alignment showed Pm-TIMP had the highest (41%) identity to that from Crassostrea gigas. Tissue expression analysis indicated Pm-TIMP was highly expressed in nacre formation related-tissues, including mantle and pearl sac. After decreasing Pm-TIMP gene expression by RNA interference (RNAi) technology in the mantle pallium, the inner nacreous layer of the shells showed a disordered growth. These results indicated that the obtained Pm-TIMP in this study participated in nacre formation. (C) 2014 Elsevier Inc. All rights reserved.