文献类型：期刊文献

英文题名：Nanoplastics aggravate the toxicity of arsenic to AGS cells by disrupting ABC transporter and cytoskeleton

作者：Lin, Peichun[1,2];Guo, Yitao[1];He, Lei[1];Liao, Xiuchun[1];Chen, Xueru[1];He, Liuying[1];Lu, Zifan[1,2];Qian, Zhong-Ji[1,2];Zhou, Chunxia[2,3];Hong, Pengzhi[2,3];Sun, Shengli[1];Li, Chengyong[1,2]

机构：[1]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Peoples R China;[3]Guangdong Ocean Univ, Sch Food Sci & Technol, Zhanjiang 524088, Peoples R China

年份：2021

卷号：227

外文期刊名：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

收录：SCI-EXPANDED(收录号：WOS:000708564700003)、、Scopus(收录号：2-s2.0-85116568177)、WOS

基金：This work was supported by Project of Enhancing School with Innovation of Guangdong Ocean University, China (2020ZDZX2029) , Science and Technology Planning Project of Zhanjiang City, China (2018A02014) , Shenzhen Science and Technology R&D Fund, China (KCXFZ202002011011057) , Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New Dis-trict, China (KY20180202, PT202001-18) , Innovation and Development Project about Marine Economy Demonstration of Zhanjiang City, China (2017C8B1) , Training Programs of Innovation and Entrepreneurship for Undergraduates, China (CXXL2020296) .

语种：英文

外文关键词：Nanoplastics; Arsenic; AGS cells; ABC transporter; Cytoskeleton

外文摘要：The coexistence of nanoplastics (NPs) and pollutants such as arsenic (As) has become an unignorable environmental problem. However, there is still a considerable knowledge gap about the impact of NPs and pollutants on human health risks. In this study, the human gastric adenocarcinoma (AGS) cells were used as a model to investigate the toxicity of NPs with different particle sizes and As by MTT assay, western blotting, immunofluorescence and so on. The results showed that 20 nm (8 mu g/mL), 50 nm (128 mu g/mL), 200 nm (128 mu g/mL), 500 nm (128 mu g/mL), 1000 nm (128 mu g/mL) polystyrene (PS) did not affect cell viability, ROS, intracellular calcium and activate apoptosis pathway in AGS cells. However, noncytotoxic concentration of NPs enhanced the cytotoxicity and intracellular accumulation of As. NPs destroys the fluidity of cell membrane and cytoskeleton, inhibits the activity of ABC transporter, and leads to the accumulation of As in cells. This work highlights that the damage caused by NPs, especially at the level of noncytotoxicity, joint with As cannot be ignored and provides a specific toxicological mechanism of NPs accompanied by exposure to As.