文献类型：期刊文献

英文题名：Effect of vitamin D3 on immunity and antioxidant capacity of pearl oyster Pinctada fucata martensii after transplantation: Insights from LC-MS-based metabolomics analysis

作者：Yang, Chuangye[1,2];Du, Xiaodong[1,2];Hao, Ruijuan[1];Wang, Qingheng[1,2];Deng, Yuewen[1,2];Sun, Ruijiao[3]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Pearl Breeding & Proc Engn Technol Res Ctr Guangd, Zhanjiang 524088, Peoples R China;[3]Zhejiang Hengxing Food Co Ltd, Jiaxing 314100, Peoples R China

年份：2019

卷号：94

起止页码：271

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000496892400030)、、WOS

基金：This work was supported by "Innovation Team Project" (Grant number: 2017KCXTD016) from the Department of Education of Guangdong Province, the Science and Technology Department, Guangdong Province (Grant number: 2018A030310666 and 2014A020208122), and the National Natural Science Foundation of China (Grant numbers: 31672626). LC-MS analysis was assisted by Biotree Biotech Co., Ltd. (Shanghai, China).

语种：英文

外文关键词：Pinctada fucata martensii; Liquid chromatography-mass spectrometry (LC-MS); Vitamin D3; Metabolomics

外文摘要：Postoperative care is a critical step of pearl culture that ultimately determines culture success. To determine the effect of dietary vitamin D3 (VD3) levels on immunity and antioxidant capacity of pearl oyster Pinctada fucata martensii during postoperative care and explore the mechanisms behind this phenomenon, five isonitrogenous and isolipidic experimental diets were formulated by adding different levels of dietary VD3 (0, 500, 1000, 3000, and 10000 IU/kg), and the diets were fed to five experimental groups (EG1, EG2, EG3, EG4, and EG5) in turn and cultured indoors. The control group (CG) was cultured in the natural sea. Pearl oysters that were 1.5 years old were subjected to nucleus insertion. After culturing for 30 days, EG3 exhibited significantly higher survival rates than those in CG and EG5 (P < 0.05). Moreover, EG3 exhibited the highest activities of alkaline phosphatase, acid phosphatase, catalase, superoxide dismutase, and lysozyme. However, EG5 achieved the highest activities of glutathione peroxidase. Metabolomics-based profiling of pearl oysters fed with high levels of dietary VD3 (EG5) and optimum levels of dietary VD3 (EG3) revealed 76 significantly differential metabolites (SDMs) (VIP > 1 and P < 0.05). Pathway analysis indicated that SDMs were involved in 21 pathways. Furthermore, integrated key metabolic pathway analysis suggested that pearl oysters in EG5 regulated the pentose phosphate pathway, glutathione metabolism, sphingolipid metabolism, and arachidonic acid metabolism in response to stress generated from excessive VD3. These findings had significant implications on strengthening the future development and application of VD3 in aquaculture of pearl oyster P. f martensii.