文献类型：期刊文献

英文题名：Polystyrene nanoplastics promote the apoptosis in Caco-2 cells induced by okadaic acid more than microplastics

作者：Yan, Linhong[1];Yu, Zihua[1];Lin, Peichun[2];Qiu, Shijie[2];He, Liuying[2];Wu, Zijie[1];Ma, Lihua[1];Gu, Yanggao[2];He, Lei[2];Dai, Zhenqing[3];Zhou, Chunxia[1];Hong, Pengzhi[1];Li, Chengyong[2,3]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Engn Technol Res Ctr Seafood, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Guangdong Prov Engn Lab Marine Biol Prod,Coll Food, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[3]Shenzhen Inst Guangdong Ocean Univ, Shenzhen 518108, Peoples R China

年份：2023

卷号：249

外文期刊名：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

收录：SCI-EXPANDED(收录号：WOS:000975932700001)、、Scopus(收录号：2-s2.0-85143486423)、WOS

基金：This work was supported by National Natural Science Foundation of China (21874068) , Guangdong Basic and Applied Basic Research Foundation (2022B1515020091, 2021A1515010291) , Shenzhen Science and Technology Program (KCXFZ202002011011057, JCYJ20210324122606017, GJHZ20210705142000003) , the Project of Enhancing School with Innovation of Guangdong Ocean University (2020ZDZX2029) , Training Programs of Innovation and Entrepreneurship for Undergraduates (CXXL2018008 , CXXL2022176) . The graphical abstract was drawn by Figdraw ( www.figdraw.com ) .

语种：英文

外文关键词：Nanoplastics; Microplastics; Okadaic acid; Caco-2 cells; Endoplasmic reticulum stress; Apoptosis

外文摘要：Microplastics (MPs) are widespread in the environment and can be ingested through food, water, and air, posing a threat to human health. In addition, MPs can have a potential combined effect with other toxic compounds. Polystyrene (PS) has been shown to enhance the cytotoxicity of okadaic acid (OA). However, it remains unclear whether this enhancement effect is related to the size of PS particles. In this study, we investigated the mechanism of the combined effect of PS microplastics (PS-MPs) or PS nanoplastics (PS-NPs) and OA on Caco-2 cells. The results indicated that PS-NPs enhanced the cytotoxicity of OA and induced endoplasmic reticulum (ER) stress-mediated apoptosis in Caco-2 cells, compared to PS-MPs. Specifically, PS-NPs and OA cause more severe oxidative stress, lactate dehydrogenase (LDH) release, and mitochondrial membrane depolarization. Furthermore, it induced intracellular calcium overload through store-operated channels (SOCs) and activated the PERK/ ATF-4/CHOP pathway to cause ER stress. ER stress promoted mitochondrial damage and finally activated the caspase family to induce apoptosis. This study provided an indirect basis for the assessment of the combined toxicity of MPs or NPs with OA.