文献类型：期刊文献

英文题名：Long-term thermal stress induces hepatic injury and alters the thermotolerance response in Hong Kong catfish (Clarias fuscus)

作者：Duan, Cunyu[1,2];Tian, Changxu[1,2];Guan, Yingyi[1];Xu, Hongfei[3];Yang, Lei[1,2];Chen, Yu[1,2];Liu, Yong[1,2];Shen, Yijun[1,2];Zhang, Yulei[1];Cao, Shouxiong[3];Huang, Yang[1,2];Li, Guangli[1,2]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Res Ctr Reprod Control & Breeding Techno, Guangdong Prov Engn Lab Mariculture Organism Breed, Guangdong Prov Key Lab Pathogen Biol & Epidemiol A, Zhanjiang 524088, Peoples R China;[3]Guangxi Intro & Breeding Ctr Aquaculture, Nanning 530001, Peoples R China

年份：2024

卷号：590

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:001241269900003)、、WOS

基金：This research was funded by Guangdong Basic and Applied Basic Research Foundation (2021A1515010733) , the Department of Education of Guangdong Province (2023KTSCX042) , the Self-financing Project of Guangxi Agricultural Science and Technology (Z2019123) , the Modern Seed Industry Park for Whiteleg Shrimp of Guangdong Province (K22221) , and the Undergraduate Innovation Team Project of Guangdong Ocean University (CXTD2023003) .

语种：英文

外文关键词：Catfish; Heat stress; Oxidative stress; Histopathology; Transcriptome

外文摘要：Global climate change has a significant impact on fish survival and the aquaculture industry. Even highly adaptable organisms like Siluriformes fish are susceptible to these effects. This study investigates the consequences of extreme high temperature on Hong Kong catfish (Clarias fuscus), a species known for its long-term habitation in tropical and subtropical regions. In this study, C. fuscus were cultivated for 90 days at high temperature (HT, 34 degrees C) and normal temperature (NT, 26 degrees C), followed by subjecting the two groups to high temperature stress (34 degrees C) and temperature recovery (26 degrees C). The hepatic histology, biochemical, and transcriptomic characteristics of the species were examined before acute high temperature stress (NT-C, HT-C), after acute high temperature stress (NT-T, HT-T), and after temperature recovery (NT-R, HT-R). The histological analysis revealed that long-term heat stress damaged the liver tissue of C. fuscus. Furthermore, significant damage was observed in the liver tissues of the NT group under acute high temperature stress, while no further damage was observed in the HT group. The biochemical analysis showed that 90 days of heat stress altered the body's immune and oxidative balance, resulting in an increase in alkaline phosphatase (ALP), alanine aminotransferase (ALT), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities, and a decrease in malondialdehyde (MDA) content. Upon acute heat stress, the liver function of the NT group was disordered and unable to produce enough total-bilirubin (T-BIL), while the aspartate aminotransferase (AST) activity of the HT group was more susceptible to being affected. Long-term heat stress led to significant changes in gene expression, with the NT group showing three times more differentially expressed genes (DEGs) identified in the liver compared to the HT group under acute heat stress conditions. Analysis of DEG enrichment during the same treatment period in both groups revealed significant differences in amino acid metabolism and lipid metabolism pathways. These results suggest that long-term heat stress caused liver tissue damage in C. fuscus, altering immune and oxidative balance, enzyme activity sensitivity, as well as the body's response to acute heat stress pathways and intensity. This study lays the groundwork for future investigations into fish adaptability to sudden temperature changes and aquaculture strategies to mitigate heat stress in fish facing extreme temperatures.