文献类型：期刊文献

中文题名：牡蛎及其酶解产物抗皮肤光老化的初步研究

英文题名：Preliminary study on resistance of oyster and its enzymatic hydrolysis products to skin photoaging

作者：吕佳桐[1];林海生[1,2];秦小明[1,2];章超桦[1,2];曹文红[1,2];高加龙[1,2];郑慧娜[1,2]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]广东省水产品加工与安全重点实验室/广东普通高等学校水产品深加工重点实验室/国家贝类加工技术研发分中心(湛江)/南海生物资源开发与利用协同创新中心,广东湛江524088

年份：2021

卷号：17

期号：1

起止页码：91

中文期刊名：南方水产科学

外文期刊名：South China Fisheries Science

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：现代农业产业技术体系建设专项资金(CARS-49);国家重点研发计划项目“水产品非宏量营养素的健康功效解析”(2018YFD0901105)。

语种：中文

中文关键词：牡蛎;酶解产物;皮肤;光老化;抗氧化;紫外线辐射

外文关键词：Oyster;Enzymatic hydrolysis product;Skin;Photoaging;Antioxidant;Ultraviolet radiation

中文摘要：文章探讨了香港牡蛎(Crassostrea hongkongensis)肉粉(Oyster meat,OM)及其酶解产物(Oyster hydrolysis,OH)对紫外线诱导的小鼠(Mus musculus)皮肤光老化的保护作用。紫外线(UVA+UVB)每天照射构建小鼠皮肤光老化模型并灌胃OM和OH,分为低、中、高三组(30、90、180 mg·kg^(?1))给药,持续8周。结果表明,OM和OH可减轻小鼠皮肤皱纹,提高皮肤弹性,减少皮下血管的萎缩,防止血管通透性增加;皮肤HE染色结果表明,中高剂量的OM和OH可显著减缓表皮层过度角化增厚(P<0.05);Masson染色和醛品红染色结果表明,OM和OH可减少胶原纤维的卷曲和降解,避免弹性纤维的异常增生,恢复真皮细胞外基质网络的排列;与模型组相比,OM和OH可使皮肤中超氧化物歧化酶(SOD)和谷胱甘肽过氧化氢酶(GSH-Px)的活力显著提高,同时使丙二醛(MDA)和8-羟基脱氧鸟苷(8-OHDG)的浓度降低(P<0.05);OM和OH可抑制基质金属蛋白酶(MMP-3、MMP-9)的表达,显著降低真皮中羟脯胺酸(Hyp)的降解;此外,高剂量的OH可显著降低皮肤中炎症因子细胞白介素6(IL-6)和肿瘤坏死因子(TNF-α)的表达,并通过提高转化生长因子(TGF-β)来提高皮肤抗炎能力。

外文摘要：To explore the protective effects of Hong Kong oyster(Crassostrea hongkongensis)meat(OM)and its enzymatic hydrolysis(OH)products on UV-induced photoaging of mouse(Mus musculus)skin.Ultraviolet rays(UVA+UVB)were irradiated every day to build a mouse skin photoaging model.The mice were given OM and OH daily and divided into low,medium and high dose groups(30,90,180 mg·kg^(?1))for eight weeks.The results show that OM and OH can reduce mice skin wrinkles,improve skin elasticity,reduce subcutaneous blood vessel atrophy,and prevent increase in vascular permeability.The results of skin HE staining show that medium and high doses of OM and OH can slow down hyperkeratosis and epidermis thickening significantly(P<0.05).Masson staining and aldehyde fuchsin staining results show that OM and OH can reduce the curling and degradation of collagen fibers,avoid abnormal proliferation of elastic fibers,and restore the arrangement of the dermal extracellular matrix network.Compared with the model group,OM and OH can increase the enzyme activity of superoxide dismutase(SOD)and glutathione catalase(GSH-Px)in skin,while malondialdehyde(MDA)and 8-hydroxydeoxyguanosine(8-OHDG)concentration decreased significantly(P<0.05).OM and OH can inhibit the expression of matrix metalloproteinases(MMP-3,MMP-9),and reduce the degradation of hydroxyproline(Hyp)in the dermis significantly.In addition,high doses of OH can reduce the expression of inflammatory factors,interleukin 6(IL-6)and tumor necrosis factor(TNF-α)in the skin significantly,and increase the skin's anti-inflammatory ability by increasing transforming growth factor(TGF-β).