文献类型：期刊文献

英文题名：Cottonseed protein concentrate (CPC) suppresses immune function in different intestinal segments of hybrid grouper female Epinephelus fuscoguttatus x male Epinephelus lanceolatu via TLR-2/MyD88 signaling pathways

作者：Yin, Bin[1,2];Liu, Hongyu[1,2];Tan, Beiping[1,2];Dong, Xiaohui[1,2];Chi, Shuyan[1,2];Yang, Qihui[1,2];Zhang, Shuang[1,2];Chen, Liqiao[3]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Anim Nutr & Feed, Zhanjiang 524025, Peoples R China;[2]Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Tech, Zhanjiang 524025, Peoples R China;[3]East China Normal Univ, Coll Life Sci, Shanghai 200062, Peoples R China

年份：2018

卷号：81

起止页码：318

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000443665900037)、、WOS

基金：This work was supported by the National Natural Science Foundation of China (No. 31772864), the National Basic Research Program of China (2014CB138600), China Agriculture Research System (CARS-47), Natural Science Foundation of Guangdong Province (2018A030313154), Zhanjiang Science and Technique Foundation (2016A03011), Project of Enhancing School With Innovation of Guangdong Ocean University (2015KTSCX055) and Hunan Xinrui Biological Technology Co.Ltd.

语种：英文

外文关键词：female Epinephelus fuscoguttatus x male Epinephelus lanceolatu; Cottonseed protein concentrate; Fish meal; Antioxidant capacity; Intestinal segments; TLR-2/MyD88 signaling

外文摘要：Cottonseed protein concentrate (CPC) has similar amino acid composition compared with fish meal, and has the characteristics of low gossypol and low toxicity. The present study was conducted to investigate the growth performance, antioxidant capacity and different intestinal segments immune responses of hybrid grouper to replacement dietary fish meal ofCPC. Six iso-nitrogenous (50% crude protein) and iso-lipidic (10% crude lipid) diets were formulated: a reference diet (FM) containing 60% fishmeal and five experimental diets (12%, 24%, 36%, 48 and 60%) in which fishmeal protein was substituted at different levels by CPC to feed fish (initial body weight: 11 +/- 0.23 g) for 8 weeks. Thena challenge test with injection of Vibrio parahaemolyticus was conducted for 7 days until the fish stabilized. The results showed that specific growth rate (SGR) was the highest with 24% replacement level and feed conversion ratio (FCR)was significantly increased when the replacement level reached 48% (P < 0.05). The content of malonaldehyde (MDA) in the serum was significantly increased when the replacement level reached 36% (P < 0.05). The plica height in the proximal, mid and distal intestine were significantly decreased with the replacement level up to 48% (P < 0.05). Hepatic fat deposition wasag-gravatedwhen the replacement level reached 36% (P < 0.05). The expression of IL-6, TNF-alpha, and IL-1 beta mRNAs were significantly up-regulated (P < 0.05). The hepcidin mRNA expression was significantly down-regulated (P < 0.05). In proximal intestine (PI) and mid intestine (MI), IFN-gamma mRNA expression was significantly upregulated (P < 0.05). These results suggested that the CPC decreased hybrid grouper growth performance and inflammation function, and different inflammation function responses in PI,MI, and distal intestine (DI) were mediated partly by the TLR-2/MyD88 signaling pathway. According to the analysis of specific growth rate, the dietary optimum replacement level and maximum replacement level were estimated to be 17% and 34%, respectively.