文献类型：期刊文献

英文题名：The effects of 17α-methyltestosterone on gonadal histology and gene expression along hypothalamic-pituitary-gonadal axis, germ cells, sex determination, and hypothalamus-pituitary-thyroid axis in zebrafish (Danio rerio)

作者：Li, Minchun[1];Zhang, Ning[2];Huang, Yiting[1];Pan, Chang-Gui[3];Dong, Zhongdian[2];Lin, Zhong[1,4];Li, Chengyong[1,4];Jiang, Yu-Xia[5];Liang, Yan-Qiu[1,4,6]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Coll Fishery, Zhanjiang, Peoples R China;[3]Guangxi Univ, Guangxi Lab Study Coral Reefs South China Sea, Nanning, Peoples R China;[4]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen, Peoples R China;[5]Minist Ecol & Environm, South China Inst Environm Sci, Guangzhou, Peoples R China;[6]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China

年份：2024

卷号：39

期号：3

起止页码：1494

外文期刊名：ENVIRONMENTAL TOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:001109322300001)、、EI(收录号：20234715100667)、Scopus(收录号：2-s2.0-85177598759)、WOS

基金：The authors would like to acknowledge the financial support from the Natural Science Foundation of Guangdong Province (2022A1515012107 and 2019A1515011889), Characteristic Innovation Projects of Ordinary Universities of Guangdong Province (2021KTSCX043), Scientific Research Fund for South China Sea Scholars Program of Guangdong Ocean University (002029002011) and Fundamental Research Funds for the Central Public Welfare Research Institutes (PM-zx703-202104-068).

语种：英文

外文关键词：17 alpha-methyltestosterone; androgen; histology; transcriptional expression; zebrafish

外文摘要：As a synthetic androgen, 17 alpha-methyltestosterone (MT) is widely used in aquaculture to induce sex reversal and may pose a potential risk to aquatic organisms. This ecological risk has attracted the attention of many scholars, but it is not comprehensive enough. Thus, the adverse effects of MT on zebrafish (Danio rerio) were comprehensively evaluated from gonadal histology, as well as the mRNA expression levels of 47 genes related to hypothalamic-pituitary-gonadal (HPG) axis, germ cell differentiation, sex determination, and hypothalamus-pituitary-thyroid (HPT) axis. Adult zebrafish with a female/male ratio of 5:7 were exposed to a solvent control (0.001% dimethyl sulfoxide) and three measured concentrations of MT (5, 51 and 583 ng/L) for 50 days. The results showed that MT had no significant histological effects on the ovaries of females, but the frequency of late-mature oocytes (LMO) showed a downward trend, indicating that MT could induce ovarian suppression to a certain extent. The transcriptional expression of activating transcription factor 4b1 (atf4b1), activating transcription factor 4b2 (atf4b2), calcium/calmodulin-dependent protein kinase II delta 1 (camk2d1), calcium/calmodulin-dependent protein kinase II delta 2 (camk2d2) and calcium/calmodulin-dependent protein kinase II inhibitor 2 (camk2n2) genes in the brain of females increased significantly at all treatment groups of MT, and the mRNA expression of forkhead box L2a (foxl2) and ovarian cytochrome P450 aromatase (cyp19a1a) genes in the ovaries were down-regulated by 5 and 583 ng/L group, which would translate into inhibition of oocyte development. As compared to females, MT had relatively little effects on the reproductive system of males, and only the transcriptional alterations of synaptonemal complex protein 3 (sycp3) and 17-alpha-hydroxylase/17,20-lyase (cyp17) genes were observed in the testes, not enough to affect testicular histology. In addition, MT at all treatments strongly increased corticotropin-releasing hormone (crh) transcript in the brain of females, as well as deiodinase 2 (dio2) transcript in the brain of males. The paired box protein 8 (pax8) gene was significantly decreased at 51 or 583 ng/L of MT in both female and male brains. The above results suggest that MT can pose potential adverse effects on the reproductive and thyroid endocrine system of fish.