文献类型：期刊文献

中文题名：凡纳滨对虾肌肉蛋白质双向电泳分离技术的建立及优化

英文题名：Establishment and Optimization of Two-dimensional Electrophoresis(2-DE) Technique in Muscle Proteome of Litopenaeus vannamei

作者：梁光明[1];庞欢瑛[2];王雅玲[1];孙力军[1];刘阳[3];徐德峰[1];陈进军[1];励建荣[4]

机构：[1]广东省水产品加工与安全重点实验室广东普通高等学校水产品深加工重点实验室广东海洋大学食品科技学院,广东湛江524088;[2]广东海洋大学水产学院广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524088;[3]国家海产品质量监督检验中心,广东湛江524000;[4]渤海大学辽宁省食品安全重点实验室,辽宁锦州121013

年份：2015

卷号：15

期号：10

起止页码：240

中文期刊名：中国食品学报

外文期刊名：Journal of Chinese Institute Of Food Science and Technology

收录：CSTPCD、、北大核心2014、EI(收录号：20160301816242)、CSCD2015_2016、Scopus(收录号：2-s2.0-84954104047)、北大核心、CSCD

基金：国家自然科学基金项目(31171634;31371777);"十二五"国家科技支撑计划项目(2012BAD29B06)

语种：中文

中文关键词：凡纳滨对虾;肌肉;双向电泳

外文关键词：Litopenaeus vannamei; meat; two-dimensional electrophoresis;

中文摘要：建立凡纳滨对虾肌肉组织双向电泳分离技术并对其进行优化。通过液氮研磨和液氮研磨＋超声破碎的方式提取对虾肌肉组织蛋白质。采用7 cm的IPG胶条进行双向电泳,凝胶染色后进行图谱分析,并对裂解液配方、胶条pH值、蛋白质上样量、凝胶染色进行优化。结果显示,以液氮研磨＋超声破碎,裂解液Ⅱ提取对虾肌肉蛋白质;采用pH 4-7 IPG胶条,180μg蛋白质上样量,并用考马斯亮蓝G-250胶体考染（蓝银）染色,成功得到背景清晰,分辨率较高的凡纳滨对虾肌肉蛋白质双向电泳图谱,这为凡纳滨对虾肌肉蛋白质组学分析及肌肉品质变化的分子标记识别提供基础。

外文摘要：Two-dimensional electrophoresis（2-DE） method on proteome of Litopenaeus vannamei was established and optimized. The shrimp proteins were extracted by grinding in liquid nitrogen and following by ultrasonication, separated by 2-DE with the 7 cm IPG strip. Image analysis software was applied to analyze the 2-DE images after staining. Effect of the lysis buffer, protein extraction method, pH of IPG gel strip, loading protein amount and stain method on 2-DE images were detected. The results showed that the 2-DE pattern of shrimp protein with clear background and better resolution was successfully obtained using lysis buffer Ⅱ, grinding in liquid nitrogen and following by ultrasonication, 7 cm pH 4-7 IPG gel strips, loading the 180 μg protein and dying the gels stained by blue silver. The study would provide a technical reference for the proteomic analysis and molecular marker identification of Litopenaeus vannamei muscle.