文献类型：期刊文献

英文题名：Response to different dietary carbohydrate and protein levels of pearl oysters (Pinctada fucata martensii) as revealed by GC–TOF/MS-based metabolomics

作者：Yang, Chuangye[1]; Hao, Ruijuan[1]; Du, Xiaodong[1,2]; Wang, Qingheng[1,2]; Deng, Yuewen[1,2]; Sun, Ruijiao[3]

机构：[1] Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China; [2] Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; [3] Zhejiang Hengxing Food Co., Ltd., Jiaxing, 314100, China

年份：2019

卷号：650

起止页码：2614

外文期刊名：Science of the Total Environment

收录：EI(收录号：20184105914063)、Scopus(收录号：2-s2.0-85054313970)

语种：英文

外文关键词：Amino acids - Disaster prevention - Disasters - Carbohydrates - Plants (botany) - Molluscs - Phosphatases - Shellfish - Metabolites - Superoxide dismutase

外文摘要：Land-based culturing can avoid the effects of environmental pollution and natural disasters, thus ensuring food safety for shellfish. However, food availability, in this case, is limited. To achieve the optimum balance of dietary carbohydrates and proteins and explore the mechanisms behind the phenomenon, we formulated five isoenergetic and isolipidic diets (C30P40, C35P35, C40P30, C45P25, and C50P20) with different levels of carbohydrates (C) and proteins (P). There were five experimental groups (C30P40, C35P35, C40P30, C45P25, and C50P20) and two control groups (CG1 and CG2). CG1 was fed with mixed powders of yeast and Chlorella sp., and CG2 was cultured in natural sea. After 60-day feeding, the highest rates of survival and absolute growth appeared in C45P25. C45P25 exhibited significantly higher activities of amylase, protease, alkaline phosphatase, acid phosphatase, superoxide dismutase, catalase, glutathione peroxidase, and phenoloxidase and significantly lower malondialdehyde content than C30P40, C35P35, C40P30, C50P20, and CG1. No significant differences were observed between C45P25 and CG2. Furthermore, the total antioxidant capacity of the pearl oysters in C45P25 was significantly higher than that in C30P40, C35P35, C40P30, and C50P20. On the basis of these results, the optimal balance of proteins and carbohydrates for pearl oysters was the C45P25 diet. Metabolomics-based profiling of the pearl oysters fed with high-carbohydrate/low-protein diet (C45P25) and low-carbohydrate/high-protein diet (C30P40) revealed 80 significantly different metabolites (VIP > 1 and P ? 2018 Elsevier B.V.