文献类型：期刊文献

英文题名：Expression and functional characterization of the mannose receptor (MR) from Nile tilapia (Oreochromis niloticus) in response to bacterial infection

作者：Yin, Xiaoxue[1];Bai, Hao[1];Mu, Liangliang[1];Chen, Nuo[1];Qi, Weiwei[1];Huang, Yu[2];Xu, Heyi[1];Jian, Jichang[2];Wang, Anli[1];Ye, Jianmin[1]

机构：[1]South China Normal Univ, Sch Life Sci, Inst Modern Aquaculture Sci & Engn, Guangdong Prov Key Lab Hlth & Safe Aquaculture, Guangzhou 510631, Peoples R China;[2]Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Guangdong South China Sea Key Lab Aquaculture Aqu, Zhanjiang 524088, Peoples R China

年份：2022

卷号：126

外文期刊名：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000703863800004)、、WOS

基金：This project was supported by National Natural Science Foundation of China (31902396, 32102826, 31972818), China Postdoctoral Science Foundation (2019M652942), Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals (KFKT2019YB07, KFKT2019YB02), Guangdong Ocean University, Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation ("Climbing Program" Special Funds, pdjh 2021b0138). Provincial Training Program of Innovation and Entrepreneurship for Undergraduates (S202010574078).

语种：英文

外文关键词：Oreochromis niloticus; C-type lectin; Mannose receptor; Pathogenic bacteria; Phagocytosis

外文摘要：Mannose receptor (MR) as a member of the pattern recognition receptors (PRRs) plays an important role in the immune response. In mammals, the role of MR in the regulation of phagocytosis is clarified; however, its contribution to opsonize phagocytosis remains unclear in bony fish. In this study, the expression pattern of Nile tilapia mannose receptor gene (OnMR) was investigated and its regulation of the phagocytosis of monocytes/ macrophages to pathogenic bacteria was identified. The full-length of OnMR open reading frame is 4314 bp, encoding a peptide containing 1437 amino acid residues. The deduced amino acid sequence revealed that OnMR contained a cysteine-rich domain, a fibronectin type II domain, multiple C-type lectin-like domains, a transmembrane domain and a short cytoplasmic domain. Tissue distribution analysis showed the OnMR transcripts was widely distribute in the ten detected tissues, and highly expressed in head kidney, hind kidney, intestine and spleen. After S. agalactiae and A. hydrophila infection, the expression of OnMR in head kidney and spleen increased significantly. Moreover, the expression of OnMR in MO/Mo were also upregulated post the infection of bacteria and mannose solutions in vitro. This suggested that MR, as a mannose receptor on macrophage surface, could respond strongly to the stimulation of pathogenic bacteria. In addition, the (r)OnMR protein could effectively bind and agglutinate S. agalactiae and A. hydrophila, and regulate the phagocytic ability of monocytes/ macrophages to pathogenic bacteria. These results suggest that OnMR is involved in response against bacterial infection in Nile tilapia, and this study will help us better understand the function of MR in teleost fish.