文献类型：期刊文献

英文题名：Effects of a sulfated glycosaminoglycan from Sepia esculenta ink on transcriptional and metabolic profiles of Saccharomyces cerevisiae

作者：Lin, Zhen[1];Luo, Ping[1];Lin, Xuan[1];Chen, Yini[1];Zhang, Yu[1];Li, Fangping[1];Tan, Xiaohui[1];Liu, Huazhong[1]

机构：[1]Guangdong Ocean Univ, Coll Chem & Environm Sci, Zhanjiang 524088, Peoples R China

年份：2022

卷号：276

外文期刊名：CARBOHYDRATE POLYMERS

收录：SCI-EXPANDED(收录号：WOS:000708818500012)、、EI(收录号：20214211019778)、Scopus(收录号：2-s2.0-85116928624)、WOS

基金：This work was supported by grants from the Natural Science Foun-dation of Guangdong Province (2019A1515011102) , the Project of Application-Based Talent Training Course from Guangdong Ocean University (570319017, 571119165) . Postgraduate Education Innovation Program of Guangdong Ocean University (202146) .

语种：英文

外文关键词：Sepia esculentai ink; sulfated glycosaminoglycan; Saccharomyces cerevisiae; transcriptome; metabolome

外文摘要：Four fractions of water-extracted Sepia esculenta ink polysaccharides (SIP) were separated by dicthylaminoethy (DEAE) cellulose chromatography. The eluted fraction with the highest yield was characterized as a sulfate-rich glycosaminoglycan named SIP-IV. According to the analysis of laser scattering and refractive index signals, SIP IV was determined to be 14.4 kDa and spherical molecular conformation in salt solution. SIP-IV is composed of fucose, galactosamine, glucosamine, mannose and glucuronic acid with a molar ratio of 5.1:7.3:3.8:1:4.4, which is obviously different from reported SIPs. SIP-IV promoted yeast proliferation and intercellular antioxidant level. Based on multi-omics strategy, data of transcriptome analysis suggested that growth promotion of SIP-IV on Saccharomyces cerevisiae might be attributed to regulation of Rho protein signal transduction, nuclear autophagy and nitrogen utilization. Combined with the metabolome results, SIP-IV also re-profiled metabolism of amino acids and phospholipids in yeast cells.