文献类型：期刊文献

英文题名：Effects of T-2 toxin on digestive enzyme activity, intestinal histopathology and growth in shrimp Litopenaeus vannamei

作者：Huang, Zhanrui[1];Wang, Yaling[1];Qiu, Mei[1];Sun, Lijun[1];Deng, Yijia[1];Wang, Xiaobo[1];Bi, Siyuan[2];Gooneratne, Ravi[3];Zhao, Jian[4]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Key Lab Adv Proc Aquat Prod, Zhanjiang 524088, Peoples R China;[2]Harbin Sport Univ, Ski Teaching & Training Base Postdoctoral Res Stn, Harbin 150008, Heilongjiang, Peoples R China;[3]Lincoln Univ, Fac Agr & Life Sci, Dept Wine Food & Mol Biosci, Canterbury 7647, New Zealand;[4]Univ New South Wales, Sch Chem Engn, Sydney, NSW 2052, Australia

年份：2019

卷号：9

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：SCI-EXPANDED(收录号：WOS:000491414200002)、、WOS

基金：This study was financially supported by the National Natural Science Foundation of China (NSFC) (No. 31371777), Science and Technology Planning Project of Guangdong Province (No. 2014B020205006), Graduate Education Innovation Program of Guangdong Ocean University (No. 201831), Technology Research Program of Shenzhen (No. KYTDPT20160316161010).

语种：英文

外文摘要：T-2 toxin (T-2), a naturally occurring mycotoxin that often accumulates in aquatic animals via contaminated feed, is toxic to animals, including humans. In this study, six groups of shrimp (n = 30 shrimps/group) were given T-2 in feed at concentrations of 0-12.2 mg/kg for 20 days. T-2 accumulation, intestinal histopathology, digestive enzyme activities and subsequent effects on shrimp are reported. Compared to the control, T-2 significantly reduced weight gain, specific growth rate, and survival. The histopathology of shrimp intestine showed concentration-dependent degenerative and necrotic changes in response to dietary T-2. Progressive damage to the microstructures of shrimp intestine occurred with increasing dietary T-2 concentrations, with initial inflammation of the mucosal tissue at T-2 concentrations of 0.5 and 1.2 mg/kg, progressing to disappearance of intestinal villi and degeneration and necrosis of the submucosa at 12.2 mg/kg. Intestinal amylase and protease activities increased at low T-2 concentrations but showed significant inhibition at high concentrations; however, the opposite trend occurred for lipase activity. Collectively, these results indicate that digestive enzyme activities and mucosal structures are markedly affected by exposure to T-2, and these may have contributed to the lower survival rate of shrimp.