文献类型：期刊文献

英文题名：Molecular Characterization and Expression of Lck in Nile Tilapia (Oreochromis niloticus) in Response to Streptococcus agalactiae Stimulus

作者：Gan, Zhen[1];Wang, Bei[1];Lu, Yishan[1];Zhu, Weiwei[1];Huang, Yu[1];Jian, JiChang[1];Wu, Zaohe[2]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524025, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China

年份：2015

卷号：175

期号：5

起止页码：2376

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000350879200006)、、EI(收录号：20151000611762)、Scopus(收录号：2-s2.0-84924047311)、WOS

基金：We thank all the laboratory members for their critical reviews and comments on this manuscript. We are especially grateful for the critical comments from the anonymous reviewers. This work was supported by research grants (no. 31302226, no. 2012B020308009) from the National Natural Science Foundation of China and Science and Technology Planning Project of Guangdong Province of China.

语种：英文

外文关键词：Oreochromis niloticus; Lck; RACE; Fluorescent quantitative real-time PCR; Streptococcus agalactiae

外文摘要：Lymphocyte-specific protein tyrosine kinase (Lck) plays a critical role in effective signal transductions that are fundamental to T cell differentiation, proliferation, and effector functions. In this paper, the Lck gene of Nile tilapia, Oreochromis niloticus (designated as On-Lck), was cloned and its expression pattern under the stimulation of Streptococcus agalactiae was investigated. Sequence analysis showed important structural characteristics required for T cell receptor (TCR) signal transduction were detected in the deduced amino acid sequence of On-Lck, and the deduced genomic structure of On-Lck was similar to the known Lck. In healthy Nile tilapia, the On-Lck transcripts were mainly detected in the thymus, spleen, head kidney, and gill. When immunized with inactivated S. agalactiae, the On-Lck mRNA expression was significantly upregulated in the thymus, spleen, and head kidney. Moreover, there was a clear time-dependent expression pattern of On-Lck after immunization, and the expression reached the highest level at 48 h in the spleen and thymus and at 72 h in the head kidney, respectively. This is the first report on the expression of Lck induced by intracellular bacteria vaccination in teleosts. These findings indicated that On-Lck may play an important role in the immune response to intracellular bacteria in Nile tilapia.