文献类型：期刊文献

中文题名：汞、镉离子急性污染对紫红笛鲷肝脏金属硫蛋白的胁迫效应

英文题名：Acute Effects of Hg^(2+) and Cd^(2+) Pollution on Metallothionein in Hepatic Tissues of Lutjanus argentimaculatus

作者：陈春亮[1];马胜伟[2];王学锋[3]

机构：[1]广东海洋大学海洋资源与环境监测中心,广东湛江524088;[2]中国水产科学研究院南海水产研究所广东省渔业生态环境重点实验室,广州510300;[3]广东海洋大学水产学院,广东湛江524088

年份：2014

卷号：33

期号：8

起止页码：1493

中文期刊名：农业环境科学学报

外文期刊名：Journal of Agro-Environment Science

收录：CSTPCD、、北大核心2011、Scopus、北大核心、CSCD、CSCD2013_2014

基金：公益性行业(农业)科研专项(201403008);国家海洋公益项目(200905005-05);广东省海洋渔业科技推广专项(A201208H01);水产优青项目(SCYQ201305);校引进人才启动项目(1312033)

语种：中文

中文关键词：紫红笛鲷;生物标志物;金属硫蛋白;汞;镉

外文关键词：Lutjanus argentimaculatus; biomarker; metallothionein（MT）; mercury; cadmium

中文摘要：为分析水体中Hg2+、Cd2+急性污染对鱼体肝脏金属硫蛋白(MT)的胁迫效应,筛选其适宜的生物标志物,通过Hg2+(0.5、1、10μg·L-1)、Cd2+(5、10、100μg·L-1)单一、混合暴露96 h半静水实验,采用生物素双抗体夹心酶联免疫吸附法测定了3、12、24、48、96 h时紫红笛鲷肝组织MT的含量。结果表明,96 h内紫红笛鲷肝脏中MT含量变化为:(1)单一暴露下,汞浓度组(0.5、1、10μg·L-1Hg2+)受诱导率分别为17.9%、43.7%、51.8%;镉浓度组(5、10、100μg·L-1Cd2+)受诱导率分别为4.3%、54.7%、84.9%。(2)Hg2+-Cd2+混合暴露下,Hg1Cd(0.5μg·L-1Hg2++10μg·L-1Cd2+)、Hg2Cd(1μg·L-1Hg2++10μg·L-1Cd2+)、Hg3Cd(10μg·L-1Hg2++10μg·L-1Cd2+)三个浓度组对鱼肝脏MT的诱导率均值依次为22.8%、26.3%、51.7%;Cd1Hg(5μg·L-1 Cd2++1μg·L-1 Hg2+)、Cd2Hg(10μg·L-1 Cd2++1μg·L-1Hg2+)、Cd3Hg(100μg·L-1Cd2++1μg·L-1Hg2+)的平均诱导率分别为30.1%、26.4%、58.3%。单一、混合暴露下皆具有明显的时间-效应关系和剂量-效应关系,汞、镉离子污染物浓度的升高和暴露时间的延长,都导致鱼体肝脏MT含量显著增加;混合暴露浓度组的诱导率总体上低于单一暴露下的诱导率,反映出Hg2+-Cd2+混合状态下的拮抗作用。

外文摘要：Metallothionein（MT）plays important roles in binding and detoxifiying heavy metals. However, the responses of MT to acute pollution of Hg^2＋and Cd^2＋in water have not been clear yet. In a semi-static experiment, the levels of MT in the hepatic tissues of Lutjanus argentimaculatus were determined with enzyme-linked binding assay（ELBA）under single and combined exposure to different concentrations of Hg^2＋and Cd^2＋at 3, 12, 24, 48, 96 h. Under exposure to single metal, the levels of MT were increased by 17.9%, 43.7% and 51.8% at 0.5, 1,10 μg Hg·L^-1, and by 4.3%, 54.7%, 84.9% at 5, 10, 100 μg Cd·L^-1, respectively. Mixed exposure of Hg-Cd promoted MT levels by 22.8%,26.3%,51.7% for Hg1Cd（0.5 μg·L^-1 Hg^2＋＋10 μg·L^-1 Cd^2＋）,Hg2Cd（1 μg·L^-1 Hg^2＋＋10 μg·L^-1 Cd^2＋）,Hg3Cd（10 μg·L^-1 Hg^2＋＋10 μg·L^-1Cd^2＋）, and by 30.1%,26.4%,58.3% for Cd1Hg（5 μg·L^-1 Cd^2＋＋1 μg·L^-1 Hg^2＋）, Cd2Hg（10 μg·L^-1 Cd^2＋＋1 μg·L^-1 Hg^2＋）, Cd3Hg（100 μg·L^-1Cd^2＋＋1 μg·L^-1Hg^2＋）, respectively. In conclusion, both single and mixed exposure of Hg^2＋ and Cd^2＋ increase MT levels in the hepatic tissues, with obvious time-response and dose-response. The induction of MT is lower in the mixed treatments than in their corresponding single ones, indicating the antagonism of Hg^2＋and Cd^2＋.