文献类型：期刊文献

英文题名：Hepatoprotective effect of clam (Corbicula fluminea) protein hydrolysate on alcohol-induced liver injury in mice and partial identification of a hepatoprotective peptide from the hydrolysate

作者：Gao, Jialong[1,2,3,4,5,6];Zhang, Chaohua[1,2,3,4,5,6];Qin, Xiaoming[1,2,3,4,5,6];Cao, Wenhong[1,2,3,4,5,6];Chen, Jianping[1,2,3,4,5,6];LI, Yujin[7];Zheng, Huina[1,2,3,4,5,6];Lin, Haisheng[1,2,3,4,5,6];Chen, Zhongqin[1,2,3,4,5,6]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang, Peoples R China;[2]Natl Res & Dev Branch Ctr Shellfish Proc, Zhanjiang, Peoples R China;[3]Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China;[4]Guangdong Prov Engn Lab Marine Biol Prod, Zhanjiang, Peoples R China;[5]Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Zhanjiang, Peoples R China;[6]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian, Peoples R China;[7]Ocean Univ China, Coll Food Sci & Engn, Qingdao, Peoples R China

年份：2022

卷号：42

外文期刊名：FOOD SCIENCE AND TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000870079300016)、、Scopus(收录号：2-s2.0-85138723162)、WOS

基金：The program was supported by the Guangdong Province Innovation School Project (2020KZDZX1112) and the special funds for the Construction of Modern Agricultural Industrial Technology System (No. CRS -49) .

语种：英文

外文关键词：Corbicula fluminea; protein hydrolysates; hepatoprotective effect; alcohol -induced liver injury; peptide

外文摘要：In the current research, edible meat of freshwater clam Corbicula fluminea was subjected to enzymatic peptide extraction using animal protease. The clam protein hydrolysate (CPH) contained 45.78% essential amino acids and 44.97% hydrophobic amino acids and showed high alcohol dehydrogenase (ADH) activation in vitro. Four fractions were divided from CPH by ultrafiltration and further investigated for their hepatoprotective effects on alcohol-induced injury in mice. The 2.5-5 kDa fraction (CPH-3) showed the best effect for preventing hepatic damage caused by alcohol, as revealed by reduced serum aspartate transaminase (AST) and alanine transaminase (ALT) activities and hepatic malondialdehyde (MDA) level, the marked decrease in the hepatic triglyceride (TG) contents, and the enhanced hepatic ADH, aldehyde dehydrogenase (ALDH), and glutathione (GSH) activities. Furthermore, histopathology of CPH-3-treated mice showed less hepatic tissue damage compared to alcohol-or other CPHs-treated mice. Hence, CPH-3 was further purified by consecutive chromatographic techniques with gel filtration and reverse -phase high-performance liquid chromatography. One hepatoprotective peptide, KKLAGFLRYYS (1330.68 Da), was identified by LC-MS/MS, which exhibited strong ADH activation activity. Results suggested that CPH could be a potential nutraceutical for facilitating alcohol metabolism and preventing or ameliorating early liver injury induced by acute alcohol exposure and that the peptide KKLAGFLRYYS is one of the main bioactive components in CPH.