文献类型：期刊文献

英文题名：Insight into the allergenicity and structure changes of parvalbumin from Trachinotus ovatus induced by dense-phase carbon dioxide

作者：Qiu, Hui[1];Duan, Weiwen[1];Hu, Weicheng[2];Wei, Shuai[1,3,4];Liu, Yanan[2];Sun, Qinxiu[1];Wang, Zefu[1];Han, Zongyuan[1];Liu, Yang[1];Liu, Shucheng[1,3,4]

机构：[1]Guangdong Ocean Univ, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Guangdong Prov Engn Technol Res Ctr Seafood, Coll Food Sci & Technol,Guangdong Prov Engn Lab Ma, Zhanjiang 524088, Peoples R China;[2]Yangzhou Univ, Coll Med, Yangzhou 225109, Peoples R China;[3]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China;[4]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China

年份：2024

卷号：260

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:001176437800001)、、EI(收录号：20240515451979)、WOS

基金：This research was supported by National Natural Science Foundation of China (32272245) , Guangdong Innovation Team of Seafood Green Processing Technology (2019KCXTD011) , and Scientific Research Start-Up Funds of Guangdong Ocean University (R20048) .

语种：英文

外文关键词：Parvalbumin; Dense -phase carbon dioxide; Desensitization

外文摘要：Parvalbumin (PV) is a major allergen in fish, and traditional treatments cannot reduce its sensitization. The effects of dense -phase carbon dioxide (DPCD) treatment on the sensitization and spatial structure of PV in Trachinotus ovatus were evaluated in this study. Western blotting and indirect ELISA were used to determine the allergenicity changes and spatial conformations of PV treated by DPCD. Tris-tricine-SDS-PAGE, circular dichroism, surface hydrophobicity, endogenous fluorescence, UV spectrophotometry, free amino group, total sulfhydryl group and SEM analyses were applied to characterize PV structure. The results showed that DPCD treatment (15 MPa, 30 min, 50 C) could reduce PV -induced allergic reactions by 39-41 %, which destroyed the normal conformational epitopes and reduced the risk of PV -induced allergy. The secondary structure changed from ordered to disordered with a decreased content of alpha-helical groups, while the internal hydrophobic groups were exposed. The total sulfhydryl group content decreased significantly (P < 0.05). The surface hydrophobicity and ultraviolet absorption spectrum were enhanced, and the endogenous fluorescence peak shifted to a long wavelength. Meanwhile, the content of free amino groups increased significantly (P < 0.05). This study could provide a theoretical basis and a promising technical approach for reduction of PV allergenicities.