文献类型：期刊文献

中文题名：温度对有水保活石斑鱼代谢与鱼肉品质的影响

英文题名：Effects of temperature on metabolism function and muscle quality of grouper during process of keeping alive with water

作者：范秀萍[1];秦小明[1];章超桦[1,2];陈建平[1];朱乾峰[1];陈鹏文[1]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点试验室,广东普通高等学校水产品深加工重点试验室,湛江524088;[2]南海生物资源开发与利用协同创新中心,广州510275

年份：2018

卷号：34

期号：14

起止页码：241

中文期刊名：农业工程学报

外文期刊名：Transactions of the Chinese Society of Agricultural Engineering

收录：CSTPCD、、EI(收录号：20184406025265)、北大核心2017、Scopus、CSCD2017_2018、北大核心、CSCD

基金：广东省科技计划项目(NO：2015B020205003); 广东普通高等学校水产品高值化加工与利用创新团队建设项目(GDOU2016030503); 广东省大学生创新创业训练计划项目(CXXL2017035)

语种：中文

中文关键词：鱼;酶活性;理化性质;珍珠龙胆石斑鱼;低温保活;血清生化

外文关键词：fish;enzyme acitivity;physiochemical properties;grouper （♀Epinephelus fuscoguttatus×♂Epinephelus lanceolatus）;low temperature keeping alive;biochemical of blood serum

中文摘要：为探讨温度对有水保活珍珠龙胆石斑鱼代谢与肌肉品质的影响,该文对不同温度有水保活珍珠龙胆石斑鱼的存活率、保活前后的水质变化、血液生化指标与肌肉理化性质等进行了测定。将体质量为(503?31)g的石斑鱼暂养48 h后,以平均1~2℃/h的速度程序降温至13~14℃,分别在15,20,25℃的条件下保活。结果显示:珍珠龙胆石斑鱼在15、20和25℃下保活96 h后的存活率分别为100%,75%和17%。低温保活显著降低水体中代谢产物含量,15组与25℃组相比,珍珠龙胆石斑鱼在24~96 h内的总氨氮产量降低量在38.12%~57.76%。表明低温通过降低鱼体的呼吸代谢,减少水体中总氨氮与非离子氨氮的浓度而减缓水质恶化。休眠后鱼体受到低温应激作用血清皮质醇与血糖水平均显著上升(P<0.05)。保活72 h后,与25℃组相比,15℃处理组鱼体血清皮质醇含量显著降低,但血糖水平显著增加(P<0.05),表明低温能降低鱼体在保活过程中的应激反应,同时出现显著的高血糖症状。与新鲜的珍珠龙胆石斑鱼相比,保活72 h后,15℃处理组血清中谷丙转氨酶、谷草转氨酶和肌酸激酶活性均无显著性变化(P>0.05),乳酸脱氢酶活性、尿素氮、尿酸、总蛋白含量均显著上升(P<0.05);而甘油三酯含量显著下降(P<0.05);且肌肉中糖原与粗脂肪质量分数分别下降32.50%和36.32%,水分、粗蛋白、乳酸含量、以及持水力和p H值均无显著变化(P>0.05),总体优于25℃处理组。表明25℃保活条件下珍珠龙胆石斑鱼通过增强机体代谢酶活性,增加能量物质代谢,而导致肌肉品质下降;而在15℃保活鱼体能维持较低的代谢水平,生存能力提高,保活72 h后肌肉品质无显著变化。因此15℃适合珍珠龙胆石斑鱼的长途保活运输。该研究结果为珍珠龙胆石斑鱼的有水保活运输提供参考。

外文摘要：The aim of this paper was to investigate the effect of temperature treatment on the metabolism function and muscle quality of grouper （♀Epinephelus fuscoguttatus×♂Epinephelus lanceolatus） before and after keeping alive with water for 72 h. Groupers with body weight of 503±31 g were cultured in laboratory without feeding for 48 h, then treated by temperature reduction to 13-14 ℃ at the rate of 2 ℃/h, and then kept alive at 15, 20, 25 ℃. According to the survival rate at different temperatures, the changes of water quality, blood biochemistry, metabolic enzyme activity and muscle quality were compared before and after keeping alive for 72 h. The results indicated that the survival rates of the groups at 15, 20, 25 ℃ after preservation for 96 h were 100%, 75% and 17%, respectively. The respiration rate was decreased as the temperature dropped, low temperature preservation （15 ℃） significantly reduced the contents of metabolites in the water, and the total ammonia nitrogen （TAN） yield in the 15 ℃ group decreased by 38.12%-57.76% compared with the 25 ℃ group. The results showed that low temperature could slow the deterioration of water quality by reducing the respiratory metabolism of fish and reducing the content of TAN and non-ionic ammonia nitrogen in water. The serum cortisol and blood glucose content were increased significantly in the hibernation group （P〈0.05）. After keeping alive for 72 h, the serum cortisol concentration in the 15 ℃ group decreased significantly, while the blood glucose concentration was higher than that in the 25 ℃ group （P〈0.05）. The results showed that hypothermia could decrease the stress response and induce the “hyperglycemia symptom” of fish during the keeping alive progress. After 72 h preservation, the serum glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, and creatine kinase activities in the 15 ℃ group showed no significant change （P〉0.05）; lactate dehydrogenase activity, the level of serum usea nitrogen, uric acid, and total protein increased, while triglyceride concentration decreased significantly （P〈0.05）. The contents of glycogen and crude fat in muscle decreased by 32.50% and 36.32% respectively, while the content of moisture, crude protein, lactic acid, and water holding capacity, and pH value had no significant change in the 15 ℃ group （P〉0.05）. The muscle quality in the 15 ℃ group was better than that in the 25 ℃ group. The results suggested that after the keeping alive at 25 ℃, the metabolism function of the grouper was increased by enhancing the activity of metabolic enzymes, which led to the deterioration of muscle quality. The dormant fish could maintain low metabolic level at 15 ℃ and improve survival ability. Therefore the most appropriate temperature for keeping alive of grouper is about 15 ℃ during the long-distance transport. The results provide a reference for the transport of grouper.