文献类型：期刊文献

英文题名：Profiling of differentially expressed genes in hepatopancreas of white shrimp (Litopenaeus vannamei) exposed to long-term low salinity stress

作者：Gao, Weihua[2];Tan, Beiping[1];Mai, Kangsen[2];Chi, Shuyan[1];Liu, Hongyu[1];Dong, Xiaohui[1];Yang, Qihui[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Anim Nutr & Feed, Zhanjiang 524025, Peoples R China;[2]Ocean Univ China, Key Lab Mariculture, Minist Educ China, Qingdao 266003, Peoples R China

年份：2012

卷号：364

起止页码：186

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:000310571300027)、、Scopus(收录号：2-s2.0-84865810301)、WOS

基金：The authors would like to extend their thanks to those who have taken the time to review this paper as well and to those who helped support this research. This work was supported by grants No. NSFC 30871928 from the National Natural Science Foundation of China and 201003020 from the Special Fund for Agro-scientific Research in the Public Interest, and by GDUPS (2011) from Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme.

语种：英文

外文关键词：Litopenaeus vannamei; Hepatopancreas; Stress; Suppression subtractive hybridization; mRNA expression

外文摘要：The Pacific white shrimp, Litopenaeus vannamei, is a euryhaline crustacean capable of tolerating a wide range of ambient salinity (0.5-40 psu). To investigate the effect of long-term low salinity stress on gene expression in the hepatopancreas in shrimp, we performed suppression subtractive hybridization (SSH) in juvenile L. vannamei exposed to long-term low salinity. The shrimp (initial body weight, 0.27 +/- 0.01 g) were cultured at salinity 2 psu and salinity 30 psu for 56 days. We then constructed forward and reverse subtractive cDNA libraries. We used bioinformatics tools and vector screening to select a total of 200 (80 from forward, 120 from reverse) randomly selected clones over 100 nucleotides in length for further analysis. Nineteen contigs and 54 singletons were generated from a total of 73 consensuses. The consensuses, upon a sequence homology search using BLASTX (NCBI), revealed that 24.66% (18/73) of them had no significant match to reported sequences in the database, suggesting that they had not previously been found and that they were probably associated with stress-regulated functions. The remaining 75.34% (55/73) of the consensuses encoded proteins were matched to a wide range of functions including immune-related functions, metabolism, ribosomal activity, transfer activity, and apoptosis. The most common group in these SSH libraries was immune-related proteins and enzymes (11/17). Quantitative RT-PCR results confirmed that the relative expression of 5 differentially expressed genes encoding hemocyanin, chitinase, ecdysteroid-regulated protein, trypsin and chymotrypsin 1 was decreased 2-, 1.45-, 11.11-, 1.33- and 1.54-fold, respectively, in the reverse library. This subtractive cDNA library provides a basis for the study of the genetic response of shrimp to environmental stress. (C) 2012 Elsevier B.V. All rights reserved.