文献类型：期刊文献

英文题名：Rapid detection of Listeria monocytogenes using fluorescence immunochromatographic assay combined with immunomagnetic separation technique

作者：Li, Qianru[1,2];Zhang, Sai[3];Cai, Yanxue[1];Yang, Yuexi[3];Hu, Fei[3];Liu, Xiaoyun[3];He, Xiaowei[1]

机构：[1]South China Univ Technol, Coll Food Sci, Guangzhou 510640, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[3]Guangzhou Wondfo Biotech Co Ltd, Natl & Local United Engn Lab Rapid Diagnost Test, Guangzhou 510663, Guangdong, Peoples R China

年份：2017

卷号：52

期号：7

起止页码：1559

外文期刊名：INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000403486300005)、、EI(收录号：20171903659595)、Scopus(收录号：2-s2.0-85019168005)、WOS

基金：This research was supported by National Science and Technology Support Program of China (No. 2012BAK08B00, 2012BAK08B07).

语种：英文

外文关键词：Fluorescence immunochromatographic; immunomagnetic beads; Listeria monocytogenes; rapid detection

外文摘要：To ensure the safety and quality of food ingredients, especially meat and dairy products, a high-throughput, rapid and sensitive method to detect Listeria monocytogenes (LM) is always on a high demand. In this work, a specific induction method to enrich and detect LM based on fluorescence immunochromatographic assay (FICA) combined with immunomagnetic separation (IMS) techniques has been developed. The immunomagnetic-beads (IM) beads were obtained through functionalised magnetic microspheres and the conjugated reaction between the carboxyl on beads surface and amino groups of antibody. The prepared IM-beads could be used for rapidly enriching pathogenic bacteria with fewer steps, resulting in a high specificity for four pathogenic serotypes and about a 40-fold improvement of detection limit compared with FICA only. In addition, this method was successfully applied in LM detection in sausage, pork and milk samples with a potential for further application in rapid on-site detection of pathogenic bacteria.