文献类型：期刊文献

中文题名：马氏珠母贝生长性状与EST-SSR标记的关联分析

英文题名：Correlation Analysis between Growth Traits and EST-SSR Markers of Pearl Oyster(Pinctada martensii)

作者：邓岳文[1];高远镇[1];王学颖[1];杜晓东[1]

机构：[1]广东海洋大学水产学院,南海水产经济动物增养殖广东普通高校重点实验室

年份：2013

卷号：21

期号：1

起止页码：77

中文期刊名：农业生物技术学报

外文期刊名：Journal of Agricultural Biotechnology

收录：CSTPCD、、北大核心2011、北大核心、CSCD、CSCD2013_2014

基金：国家科技部成果转化项目(No.2011GB2E000008);国家贝类产业体系(No.CARS-048);广东省海洋与渔业局重大科技兴海(渔)项目(No.A200908A05和No.A201008A04)

语种：中文

中文关键词：马氏珠母贝;生长性状;EST—SSS标记;关联分析;遗传多样性

外文关键词：Pearl oyster （Pinctada martensii）, Growth traits, EST-SSR markers, Correlation analysis, Geneticdiversity

中文摘要：马氏珠母贝(Pinctada martensii)生长性状属于数量性状,为了筛选与马氏珠母贝生长性状位点连锁的分子标记,本研究利用50对多态性SSR引物对选系F4的90个个体的遗传多样性进行了研究,并利用SPSS16.0软件线性模型(GLM)进行了体质量、壳长、壳高和壳宽与标记的关联分析。结果表明,50对SSR引物在90个样本中共检测到204个等位基因,等位基因数(Ne)在2～9之间,平均等位基因4.080,有效等位基因数(Na)为2.574;观察杂合度(Ho)、期望杂合度(He)和多态信息含量(PIC)分别为0.534、0.543和0.491;共检测到15个SSR标记与4个生长性状具有显著相关性(P<0.05),其他35个标记与性状的相关性未达到显著水平(P>0.05);7个SSR标记与体质量显著相关(P<0.05),6个SSR标记与壳长显著相关(P<0.05),6个SSR标记与壳高显著相关(P<0.05),4个SSR标记和壳宽显著相关(P<0.05)。对同一标记不同基因型间进行多重比较,分别找到了4个性状的优势基因型。本研究筛选了与生长性状位点连锁的标记,为后续的分子标记辅助育种提供技术支撑。

外文摘要：Growth traits of pearl oyster （Pinctada martensii） is quantitative traits. Genetic diversity of 90 individuals sampled from the fourth generation selected line of pearl oyster were evaluated by 50 EST-SSR markers, and then a correlation analysis between the markers and total weight, shell length, shell height and shell width of the selected line was performed using a GLM model of SPSS 16.0 software. The results found that total of 204 alleles were detected and the number of alleles（Ne） were between 2-9 at each locus, with 4.080 number of alleles on average. The effective number of alleles （Na） was 2.574. The mean values of observed heterozygosity （Ho）, expected heterozygosity （He） and polymorphism information content （PIC） were 0.534, 0.543 and 0.491, respectively. Totally, 15 loci were significantly related with the growth traits （P〈0.05）. There were 7 loci significantly related with total weight （P〈0.05）, 6 loci significantly related with shell length （P〈0.05）, 6 loci significantly related with shell height （P〈0.05） and 4 loci significantly related with shell width （P〈0.05）. Favorable genotypes for each growth traits were obtained by a multiple comparison among the loci. The correlation obtained in this study between growth trait and EST-SSR markers is benefit to further study in the marker assisted breeding of this species.