文献类型：期刊文献

中文题名：金钱鱼Zar1基因克隆及其表达分析

英文题名：cDNA Cloning and mRNA Expression Analysis of Zar1 in Spotted Scat(Scatophagus argus)

作者：何飞祥[1];江东能[1];陈华谱[1];邓思平[1];吴天利[1];田昌绪[1];石红娟[1];朱春华[1];李广丽[1]

机构：[1]广东海洋大学水产学院//南海水产经济动物增养殖广东省普通高校重点实验室//广东省名特优鱼类生殖调控与繁育工程技术研究中心//湛江市海洋生态与养殖环境重点实验室,广东湛江524088

年份：2019

卷号：39

期号：2

起止页码：1

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD

基金：湛江市财政资金科技竞争性分配项目(2016A03017);广东省自然科学基金(2016A030313743)

语种：中文

中文关键词：金钱鱼;Zar1;克隆;卵子发生;胚胎发育

外文关键词：Scatophagus argus;Zar1;clone;oogenesis;embryonic development

中文摘要：【目的】分析金钱鱼(Scatophagus argus)卵巢发育过程重要基因——合子阻滞因子1(Zygote arrest 1,Zar1)组织表达及其在卵子成熟和胚胎发育过程中的表达。【方法】克隆金钱鱼Zar1基因,采用反转录-PCR(RT-PCR)检测其在各组织的分布情况,实时荧光定量PCR(q PCR)研究Zar1在不同卵巢发育时期和胚胎发育过程中的表达。【结果】金钱鱼Zar1的开放阅读框(ORF)序列全长为1008bp,编码335个氨基酸。序列分析发现,金钱鱼Zar1在蛋白C末端高度保守,具有非典型的PHD基序(Plant homeo domain)和锌指结构域。金钱鱼Zar1同源性分析发现,它与鲈形目物种相似度最高,其中大黄鱼(Larimichthys crocea)相似性最高,为85.1%。系统进化树分析显示,金钱鱼Zar1与大黄鱼亲缘关系最近,与其分类地位一致。组织分布发现,金钱鱼Zar1仅在卵巢中表达。Zar1在II、III和IV期卵巢表达呈现逐渐递增趋势,其中IV期表达水平显著高于II期卵巢。Zar1在胚胎发育早期表达较高,后期较低,其中在四细胞期表达水平最高,之后逐渐降低。【结论】金钱鱼Zar1在卵巢和胚胎期表达,在金钱鱼卵子发生和胚胎发育阶段发挥重要作用。

外文摘要：【Objective】Zygote arrest 1(Zar1), as an important gene in the ovarian development, the expression of tissue distribution, and its expression during ovarian maturation and embryonic development were analyzed in spotted scat(Scatophagus argus). 【Methods】In this study, we have cloned S. argus Zar1, and analyzed its tissue distribution by RT-PCR, and expression profiles at different ovary development stages and embryonic development by quantitative real-time PCR.【Results】The open reading frame(ORF) of the Zar1 was 100 8 bp in length and encoded 335 amino acids. Sequence analysis revealed that the S. argus Zar1 was extremely conserved at the C-terminus of the amino acid, and had an atypical PHD motif and a zinc finger structure. The homology analysis showed that the gene has highest identity with those of other perciformes fishes, and the similarity with the large yellow croaker(Larimichthys crocea) was up to 85.1%. Phylogenetic tree analysis showed that the Zar1 was closest to L. crocea, which indicated consistent with its taxonomy. The tissue distribution found that the Zar1 was only expressed in the ovary. In the stages of ovary development, stage II, III and IV showed an increasing expressed trend, and Zar1 expression level at stage IV was significantly higher than that of stage II. Zar1 expression was higher in the early stages of embryonic development stages and lower in the later stages. The expression reached the highest level at the four-cell stage, and then gradually decreased. 【Conclusion】The S. argus Zar1 is expressed in the ovary and embryonic stages, it may play an important role in oogenesis and embryonic development.