文献类型：期刊文献

英文题名：Integrated analysis of microbiome and host transcriptome reveals the damage/protective mechanism of corn oil and olive oil on the gut health of grouper ( Epinephelus fuscoguttatus × E. lanceolatu)

作者：Yan, Xiaobo[1,2]; Huang, Weibin[1,2]; Suo, Xiangxiang[1,2]; Pan, Simiao[1,2]; Li, Tao[1,2]; Liu, Hao[1,2]; Tan, Beiping[1,2,3]; Zhang, Shuang[1,2,3]; Yang, Yuanzhi[1]; Dong, Xiaohui[1,2,3]

机构：[1] Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, China; [2] GuangDong Engineering Technology Research Center of Aquatic Animals Precision Nutrition and High Efficiency Feed, Guangdong, Zhanjiang, 524088, China; [3] Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Guangdong, Zhanjiang, 524000, China

年份：2023

卷号：253

外文期刊名：International Journal of Biological Macromolecules

收录：EI(收录号：20234314938406)、Scopus(收录号：2-s2.0-85174444128)

语种：英文

外文关键词：Bacteria - Cell proliferation - Cell signaling - Fish - Gene expression - Olive oil - Pathology - Pigments - Signaling

外文摘要：As digestive and immune organs of animals, the gut was frequently used to evaluate the health status of aquatic animals. In previous oil source alternatives study, corn oil (CO) had been found to induce gut inflammation, while olive oil (OO) had been found to be effective in protecting intestinal health. Three diets with different oil sources (fish oil, CO, OO) were formulated for an 8-week culture experiment, and it was proposed to combine 16S sequencing and transcriptome sequencing analysis to preliminarily elucidate the damage/protection mechanism of CO and OO on the gut health of grouper ( Epinephelus fuscoguttatus × E. lanceolatu). We found that CO indeed damaged to gut health and destroyed the gut structure, while OO had a positive outcome in protecting the gut structure, promoting digestibility and relieving enteritis. Photobacterium, Romboutsia and Epulopiscium were significantly enriched in OO group and Staphylococcus were significantly enriched in CO group. Transcriptome sequencing further revealed CO could activated Complement and coagulation cascades, Staphylococcus aureus infection, Systemic lupus erythematosus, and Tuberculosis pathways; conversely, OO activated B-cell signaling receptors, promoted B-cell proliferation and apoptosis, and thus activated B-cell signaling pathways to enhance immunity, whereas OO can regulate IL17 signaling pathway and TNF signaling pathway to inhibit NF-κB signaling pathway to reduce pro-inflammatory response. By integrating the microbiome and transcriptome, further identified all differential microorganisms were directly and significantly correlated with differential genes, and Clostridium_sensu_stricto_1, Romboutsia, Staphylococcus might as the core regulates the expression of differential gene in the organism. These results reveal that different oil sources alter gut gene expression mainly by modulating the composition and abundance of gut microbiota, further regulating the health status of the gut. Gut microbiota could be used as biomarkers to provide reference and solutions for the mitigation of inflammation in aquatic animals. ? 2023 Elsevier B.V.