文献类型：期刊文献

英文题名：Oryzias curvinotus in Sanya Does Not Contain the Male Sex-Determining Gene dmy

作者：Dong, Zhongdian[1,2,3];Li, Xueyou[1];Yao, Zebin[1];Wang, Chun[1];Guo, Yusong[1,2,3];Wang, Qian[4];Shao, Changwei[4];Wang, Zhongduo[1,2,3,5]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Guangdong South China Sea Key Lab Aquaculture Aqu, Zhanjiang 524025, Peoples R China;[2]Guangdong Ocean Univ, Fisheries Coll, Guangdong Prov Engn Lab Mariculture Organism Bree, Zhanjiang 524025, Peoples R China;[3]Guangdong Ocean Univ, Fisheries Coll, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China;[4]Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China;[5]Hunan Normal Univ Sch, Coll Life Sci, State Lab Dev Biol Freshwater Fish, Changsha 410081, Peoples R China

年份：2021

卷号：11

期号：5

外文期刊名：ANIMALS

收录：SCI-EXPANDED(收录号：WOS:000653451400001)、、Scopus(收录号：2-s2.0-85105232882)、WOS

基金：This work was funded by the National Natural Science Foundation of China (41806195, 31972794), Special innovation projects of colleges and universities in Guangdong province (2018KTSCX087, 230419055), Nanhai Scholar Project of GDOU (QNXZ201807, 201903), Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals, Guangdong Ocean University (KFKT2019ZD03) and State Laboratory of Developmental Biology of Freshwater Fish (2020KF004).

语种：英文

外文关键词：Oryzias curvinotus; dmy; sex determining gene; RNA-Seq

外文摘要：Simple Summary dmy is considered to be the male-determining gene in Japanese medaka (Oryzias latipes) and Hainan medaka (Oryzias. curvinotus), both of which have the XX/XY sex-determination system. Here, we found a group of medaka in the Sanya River (named SY-medaka) and confirmed that SY-medaka belongs to O. curvinotus by morphological characteristics and mitochondrial phylogenetic analysis. Through genetic sex identification, genome re-sequencing and gonadal transcriptome analysis, it was preliminary confirmed that SY-medaka did not contain dmy. Our results provide a basis for further studies of the mechanism underlying sex determination in Oryzias and functional genomics and reproduction biology in O. curvinotus. Hainan medaka (Oryzias curvinotus) is distributed in the coastal waters of the South China Sea and is able to adapt to a wide range of salinities. In this study, we characterized O. curvinotus in Sanya River (SY-medaka), which lacks dmy (a male sex-determining gene in O. latipes and O. curvinotus). In a comparison of SY-medaka and Gaoqiao medaka (GQ-medaka), the morphological difference between the two populations does not reach the subspecies level and they can be considered two geographic populations of O. curvinotus. A mitochondrial cytochrome oxidase subunit I (CoI) sequence alignment showed that the sequence identities between SY-medaka and other geographic populations of O. curvinotus are as high as 95%. A phylogenetic analysis of the mitochondrial genome also indicated that SY-medaka belongs to O. curvinotus. Molecular marker-based genetic sex assays and whole genome re-sequencing showed that SY-medaka does not contain dmy. Further, in RNA-Seq analyses of the testis and ovaries of sexually mature SY-medaka, dmy expression was not detected. We speculate that high temperatures resulted in the loss of dmy in SY-medaka during evolution, or the lineage has another sex-determining gene. This study provides a valuable dataset for elucidating the mechanism underlying sex determination in Oryzias genus and advances research on functional genomics or reproduction biology in O. curvinotus.