文献类型：期刊文献

中文题名：摘除眼柄与注射眼柄粗提物对凡纳滨对虾性腺发育相关基因表达的影响

英文题名：Effects of the eyestalk hormones and eyestalk ablation on the gene expreesions related gonadal development of Litopenaeus vannamei

作者：师尚丽[1,2,3];叶满[1,2,3];朱春华[1,2,3];邓思平[1,2,3];陈华谱[1,2,3];李广丽[1,2,3]

机构：[1]广东海洋大学水产学院,广东湛江524088;[2]广东海洋大学南海水产经济动物增养殖广东普通高校重点实验室,广东湛江524088;[3]广东海洋大学海洋生态与养殖环境湛江市重点实验室,广东湛江524088

年份：2016

卷号：25

期号：3

起止页码：344

中文期刊名：上海海洋大学学报

外文期刊名：Journal of Shanghai Ocean University

收录：CSTPCD、、北大核心2014、北大核心、CSCD_E2015_2016、CSCD

基金："扬帆计划"培养高层次人才项目(粤人才办〔2014〕1号);广东省海洋渔业科技与产业发展专项技术推广项目(A201408A06;B201500B02);广东海洋大学科学项目(E151811)

语种：中文

中文关键词：凡纳滨对虾;眼柄;DMC1;VASA;VTG

外文关键词：Litopenaeus vannamei; eyestalk; DMC1; VASA; VTG

中文摘要：利用荧光定量PCR技术,检测了眼柄粗提物对凡纳滨对虾(Litopenaeus vannamei)性腺发育相关基因(DMC1、VASA和VTG)表达的影响,探讨性腺抑制激素(GIH)在性腺发育过程中的调控机制,结果表明:DMC1和VASA只在凡纳滨对虾精巢和卵巢中表达;摘除眼柄后,精巢和卵巢中DMC1表达量均显著性降低(P<0.05),而注射眼柄粗提物后精巢和卵巢中DMC1表达显著增高(P<0.05);相反,摘除眼柄后,精巢和卵巢中VASA以及卵巢中VTG基因表达量显著增高,但注射眼柄粗提物后性腺中的VASA和卵巢中的VTG表达量显著降低。结果表明眼柄粗提物通过影响生殖相关基因的表达影响凡纳滨对虾的性腺发育。

外文摘要：To investigate the regulatory mechanism of gonad inhibiting hormone（ GIH） during gonadal development of Litopenaeus vannamei,expressions of gonadal development related genes（ DMC1,VASA and VTG） were detected by real time fluorescent quantitative PCR after eyestalk ablation,or injection of the eyestalk extract. The results showed that DMC1 and VASA were only expressed in the testis and ovary in L.vannamei. DMC1 mRNA expression decreased significantly in testis and ovary after eyestalk ablation（ P〈 0. 05）,while it increased significantly after eyestalk extract hormone injection（ P 〈 0. 05）. On the contrary,VTG expressions in ovaries,as well as VASA expressions in testis and ovaries increased significantly after eyestalk ablation,but decreased after eyestalk extract hormone injection. Results showed that eyestalk extract hormone affected the development of gonad by affecting the expression of the reproductive related genes.