文献类型：期刊文献

英文题名：Functional characterization of galectin-3 from Nile tilapia (Oreochromis niloticus) and its regulatory role on monocytes/macrophages

作者：Niu, Jinzhong[1,2,3];Huang, Yu[1,2,3,4,5];Liu, Xinchao[1,2,3];Luo, Guoling[1,2,3];Tang, Jufen[1,2,3,4,5];Wang, Bei[1,2,3,4,5];Lu, Yishan[1,2,3,4,5];Cai, Jia[1,2,3,4,5,6];Jian, Jichang[1,2,3,4,5]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[3]Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China;[4]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China;[5]Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen 518120, Guangdong, Peoples R China;[6]Guangxi Acad Sci, Guangxi Inst Oceanog, Guangxi Key Lab Marine Biotechnol, Beihai 536000, Peoples R China

年份：2019

卷号：95

起止页码：268

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000503099200026)、、Scopus(收录号：2-s2.0-85073833936)、WOS

基金：This work was supported by National Natural Science Foundation of China (Grant no. 31302226, 31572651), National Key R&D Program of China (2018YFD0900501), Technology Planning Project of Guangdong Province of China (grant no. 2015A020209181 and 2017A030307033), and Special foundation for "achieving the first class" of Guangdong Province (231419013).

语种：英文

外文关键词：Galectin-3; Nile tilapia; Monocytes/macrophages; Inflammation; Phagocytosis

外文摘要：Galectin-3 is a kind of beta-galactoside-binding lectin involved in host defense against pathogen infection. However, the immune functions of fish galectin-3 remain poorly understood. In this study, the roles of a fish galectin-3 (OnGal-3) from Nile tilapia (Oreochromis niloticus) on the binding activity on bacterial pathogens or PAMPs, the agglutinating activity on bacterial pathogens and the regulatory effects on monocytes/macrophages activity were investigated. After in vitro challenge of Streptococcus agalactiae and Aeromonas hydrophila, OnGal-3 expressions were significantly up-regulated in monocytes/macrophages. In addition, recombinant OnGal-3(rOnGal-3) protein showed strong binding activity on bacterial pathogens or PAMPs. Also, rOnGal-3 agglutinated Gram-positive and Gram-negative bacteria. Moreover, rOnGal-3 could induce the inflammatory factors expressions in monocytes/macrophages and enhance phagocytosis and respiratory burst activity of monocytes/ macrophages. These results suggest that fish galectin-3 participates in anti-bacterial immune response through recognizing pathogens and modulating monocytes/macrophages activity.