文献类型：期刊文献

英文题名：Purification and Identification of Peptides from Oyster (Crassostrea hongkongensis) Protein Enzymatic Hydrolysates and Their Anti-Skin Photoaging Effects on UVB-Irradiated HaCaT Cells

作者：Peng, Zhilan[1,2,3,4];Gao, Jialong[2,4,5];Su, Weimin[2,4,5];Cao, Wenhong[2,4,5];Zhu, Guoping[2,4,5];Qin, Xiaoming[2,4,5];Zhang, Chaohua[2,4,5];Qi, Yi[1,3]

机构：[1]Guangdong Med Univ, Marine Biomed Res Inst, Zhanjiang 524023, Peoples R China;[2]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[3]Marine Biomed Res Inst Guangdong Zhanjiang, Zhanjiang 524023, Peoples R China;[4]Natl Res & Dev Branch Ctr Shellfish Proc, Zhanjiang 524088, Peoples R China;[5]Guangdong Prov Engn Lab Marine Biol Prod, Zhanjiang 524088, Peoples R China

年份：2022

卷号：20

期号：12

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:000902616000001)、、WOS

基金：This research was funded from the Modern Agricultural Industry Technology Research System of China (CARS-49), the Program of Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety (GDPKLAPPS2017), and the Project of Ph.D. Start-up Fund of Guangdong Medical University (4SG22196G).

语种：英文

外文关键词：oyster; peptide; photoaging; purification; characterization

外文摘要：This study aimed to purify and identify antiphotoaging peptides from oyster (Crassostrea hongkongensis) protein enzymatic hydrolysates (OPEH) and to investigate the possible mechanism underlying its antiphotoaging effect. Multiple methods (Ultrafiltration, G25 Chromatography, RP-HPLC, and LC/MS/MS) had been used for this purpose, and eventually, two peptides, including WNLNP and RKNEVLGK, were identified. Particularly, WNLNP exerted remarkable antiphotoaging effect on the UVB-irradiated HaCaT photoaged cell model in a dose-dependent manner. WNLNP exerted its protective effect mainly through inhibiting ROS production, decreasing MMP-1 expression, but increasing extracellular pro-collagen I content. Furthermore, WNLNP downregulated p38, JNK, ERK, and p65 phosphorylation in the MAPK/NF-kappa B signaling pathway and attenuated bax over-expressions but reversed bcl-2 reduction in UVB- irradiated HaCaT cells. The molecular docking analysis showed that WNLNP forms five and seven hydrogen bonds with NF-kappa B (p65) and MMP-1, respectively. This study suggested that a pentapeptide WNLNP isolated from OPEH had great potential to prevent and regulate skin photoaging.