文献类型：期刊文献

英文题名：Identification and functional studies of Akirin, a potential positive nuclear factor of NF-kappa B signaling pathways in the Pacific white shrimp, Litopenaeus vannamei

作者：Hou, Fujun[1];Wang, Xianzong[1];Qian, Zhaoying[1];Liu, Qiao[1];Liu, Yongjie[1];He, Shulin[1];Mi, Xiao[1];Bai, Cheng[2];Sun, Chengbo[2];Liu, Xiaolin[1]

机构：[1]Northwest A&f Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Guangzhou 524088, Guangdong, Peoples R China

年份：2013

卷号：41

期号：4

起止页码：703

外文期刊名：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000326258500027)、、WOS

基金：This work was supported by the Agricultural Science and Technology Achievement Transformation Fund Project of Ministry of Science and Technology, China (no. 2012GB2E200361), the National Fundamental Research Program, China (no. 2006AA10A406), the Key Laboratory of Marine Biology, Institute of Oceanlogy, Chinese Academy of Sciences, Qianjiang distinguished experts of Hangzhou and Extension and Demonstration Program in Desalting Culture of Litopenaeus Vannamei New Breed "Ke Hai 1", Northwest A&F University, China (no. XNY2013-4). Comments from anonymous reviewers have greatly improved the manuscript.

语种：英文

外文关键词：Akirin; NF-kappa B; Antimicrobial peptides; Litopenaeus vannamei; RNA interference; Bacteria

外文摘要：As conserved nuclear factors, Akirins play critical roles in regulating antimicrobial peptides (AMPs) transcription downstream of NF-kappa B dependent signaling pathways in insects and mammals. However, no any functional studies was reported in penaeid shrimp. The identification and functional analysis of Akirin in the Pacific white shrimp, Litopenaeus vannamei were made in this research. The 833 nucleotides cDNA of Litopenaeus vannamei Akirin (LvAkirin) was obtained with an open reading frame of 639 bp, which encodes a putative protein of 212 amino acids. The molecular weight of LvAkirin is about 23.7 kDa with theoretical pI of 9.05. Two predicted nuclear localization signals (NLSs) were found and amino acid sequence alignments showed that Akirins are highly conserved between insects and mammals. The constitutive expression of LvAkirin mRNA was confirmed in all the examined tissues and high level appeared in testis followed by hemocytes and gill. LvAkirin mRNA was strongly induced in response to Vibrio parahaemolyticus infection. Silencing LvAkirin by dsRNA significantly reduced the expression of NF-kappa B dependent anti-lipopolysaccharide factor, crustin and penaeidin3a as well as transcription factors, Dorsal and Relish post Vibrio anguillarum (V. anguillarum) and Micrococcus lysodeikticus (M. lysodeikticus) challenge. Antibacterial activities of shrimp plasma was analyzed and high cumulative mortality was found in LvAkirin-silenced shrimps post bacteria challenge. Hence, we proposed LvAkirin might function as a positive nuclear factor of NF-kappa B dependent signaling pathways in shrimp innate immunity. (C) 2013 Elsevier Ltd. All rights reserved.