文献类型：期刊文献

中文题名：尼罗罗非鱼LCP2在无乳链球菌感染后的表达特征分析

英文题名：LCP2 from Nile tilapia following Streptococcus agalactiae infection

作者：张湘东[1,2];徐蔼宣[1,2];陈心怡[1,2];栗涵[1,2];夏洪丽[2];鲁义善[1,2];汪志文[1,2]

机构：[1]广东海洋大学水产学院,广东省水产动物病害防控与健康养殖重点实验室,广东湛江524088;[2]广东海洋大学深圳研究院,广东省水生动物健康评估工程技术研究中心,深圳市海水经济动物种苗健康评价公共技术服务平台,广东深圳518120

年份：2026

卷号：33

期号：2

起止页码：72

中文期刊名：中国水产科学

外文期刊名：Journal of Fishery Sciences of China

收录：北大核心2023、、北大核心

基金：广东省基础与应用基础研究基金项目(2021A1515110214);广东省普通高校特色创新类项目(2024KTSCX201).

语种：中文

中文关键词：尼罗罗非鱼;无乳链球菌;LCP2;组织表达

外文关键词：Oreochromis niloticus;Streptococcus agalactiae;LCP2;tissue expression

中文摘要：淋巴细胞胞质蛋白2(lymphocyte cytosolic protein 2,LCP2/SLP-76)作为T细胞受体(T cell receptor,TCR)信号通路的关键衔接蛋白,可有效介导B细胞、T细胞的信号转导过程,并能激活自然杀伤细胞的共刺激信号。目前,该蛋白在哺乳动物中的免疫功能已得到明确阐释,但在硬骨鱼类中的相关研究仍较为匮乏。本研究以尼罗罗非鱼(Oreochromis niloticus)为研究对象,采用无乳链球菌(Streptococcus agalactiae)感染实验结合生物信息学分析技术,对尼罗罗非鱼LCP2基因(OnLCP2)的免疫功能特征进行了初步探究。实验结果表明,克隆获得的OnLCP2基因开放阅读框全长1194 bp,编码397个氨基酸;生物信息学预测显示,其编码蛋白分子量为41.78 kDa,理论等电点pI=8.67,且该蛋白含有保守的SH2结构域(A^(10)-K^(78))和SAM基序(K^(280)-G^(396)),其三级结构与哺乳动物LCP2蛋白具有高度相似。组织表达谱分析显示,OnLCP2基因在胸腺和脾脏中的表达量最高;经无乳链球菌感染后,该基因在头肾中于感染后4 h达到表达峰值,在脾脏中于感染后24 h达到表达高峰。上述实验结果表明OnLCP2可能参与了尼罗罗非鱼抵御无乳链球菌感染的免疫应答过程,为我们进一步探究其免疫功能奠定了坚实的前期基础。

外文摘要：Lymphocyte cytosolic protein 2(lymphocyte cytosolic protein 2,LCP2/SLP-76),a critical adaptor protein in the T cell receptor(TCR)signaling pathway,mediates B-cell and T-cell receptor signal transduction and activates costimulatory signaling in natural killer cells.Although its immune functions are well-established in mammals,research on LCP2 in teleost fish remains limited.In this study,The Nile tilapia(Oreochromis niloticus)was used as the research object.Combined with Streptococcus agalactiae e infection experiments and bioinformatic analysis techniques,we preliminarily explored the immune functional characteristics of the LCP2 gene(OnLCP2)in Nile tilapia.The experimental results showed that,The cloned OnLCP2 gene contains a 1194 bp open reading frame encoding a 397-amino acid protein with a predicted molecular weight of 41.78 kDa and a theoretical isoelectric point(pI)of 8.67.Bioinformatic analysis identified a conserved SH2 domain(A^(10)-K^(78))and a SAM motif(K^(280)-G^(396))in OnLCP2 and indicated high tertiary structural similarity to mammalian LCP2.Tissue expression profiling revealed that OnLCP2 expression was highest in the thymus and spleen.Following S.agalactiae challenge,OnLCP2 expression was significantly upregulated,peaking at 4 h post-infection(hpi)in the head kidney and at 24 hpi in the spleen.The upregulation of LCP2 in immune tissues after bacterial challenge supports its role in the anti-bacterial immune response of Nile tilapia,although its precise signaling mechanisms require further investigation.The above experimental results suggest that OnLCP2 may participate in the immune response of Nile tilapia against Streptococcus agalactiae infection,which lays a solid preliminary foundation for further exploration of its immune function.