文献类型：期刊文献

英文题名：Insulin-like androgenic gland hormone from the shrimp Fenneropenaeus merguiensis: Expression, gene organization and transcript variants

作者：Zhou, Tingting[1];Wang, Wei[1];Wang, Chenggui[1];Sun, Chengbo[1,2];Shi, Lili[1];Chan, Siuming F.[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang, Guangdong, Peoples R China;[2]Nanhai Econ Shrimp Breeding & Culture Lab, Zhanjiang, Peoples R China

年份：2021

卷号：782

外文期刊名：GENE

收录：SCI-EXPANDED(收录号：WOS:000630949000003)、、Scopus(收录号：2-s2.0-85102452930)、WOS

基金：This research was funded by the National Natural Science Foundation of China (NSFC #31572606) the GDOU?s University Research Enhancement Fund (Project #2013050101, #201350109, #20135021, #2014B020205014) , Guangdong Provisional Research Grant (#2014B020202014) , a Zhanjiang Research grant (#2015A03030) , a PhD Startup Fund of Natural Science Foundation of Guangdong Province, China (No. 2016A030310334) and the Free application Fund of Natural Science Foundation of Guangdong Province, China (2018A030313963) .

语种：英文

外文关键词：Shrimp; Sex determination; Insulin-like androgenic gland hormone; Transcript variants; Alternative splicing

外文摘要：Male sex differentiation in the crustacean is best known to be controlled by the insulin-like androgenic gland hormone (IAG). In this report, the cDNA and gene of the shrimp Fenneropenaeus merguiensis FmIAG were studied and characterized. FmIAG gene shares a high sequence identity in the coding region as well as the promoter region with that of F. chinensis. FmIAG gene is most likely consists of 5 exons and 4 introns. The cDNA reported here is the most abundant transcript that retained cryptic intron 4. The use of different splicing acceptor sites in exon 2 can produce a long-form FmIAG transcript variant with 6 additional amino acids inserted. Splicing of cryptic intron 4 would produce a transcript variant with a different C-terminal end. Therefore 4 different FmIAG transcripts can be produced from the FmIAG gene. During the molt cycle, the expression level of FmIAG was low in the early intermolt, increase steadily towards the late premolt and decreased rapidly in the early postmolt. In addition to the androgenic gland, FmIAG is also expressed in the hepatopancreas and ovary of adult females. Unilateral eyestalk ablation caused a significant increase in FmIAG transcript suggesting that the eyestalk consists of inhibiting factor(s) that suppresses FmIAG expression. To explore the function of FmIAG in males, injection of FmIAG dsRNA knock-down the expression of FmIAG and up-regulated the expression of the vitellogenin gene in the testis and hepatopancreas. Interestingly a CHH-like gene identified in the androgenic gland was down-regulated. CHH-like gene knock-down resulted in altered expression of FmIAG in males suggesting that the CHH-like may be involved in FmIAGs regulation. RT-PCR with specific primers to the different transcript variant were used to determine if there is an association of different sizes of male and the type of IAG transcript. Results indicated that a high percentage of the large male shrimp expressed the long-form of FmIAG. The results suggested that FmIAG may be useful as a size marker for male shrimp aquaculture. In summary, the results of this study have expanded our knowledge of shrimp insulin-like androgenic gland hormone in male sex development and its potential role as a marker gene for growth regulation in shrimp.