文献类型：期刊文献

英文题名：An ACE inhibitory peptide from Isochrysis zhanjiangensis exhibits antihypertensive effect via anti-inflammation and anti-apoptosis in HUVEC and hypertensive rats

作者：Pei, Yu[1,2];Cai, Shengxuan[1,2];Ryu, Bomi[1,3];Zhou, Chunxia[1,2];Hong, Pengzhi[1,2];Qian, Zhong-Ji[1,2]

机构：[1]Shenzhen Inst Guangdong Ocean Univ, Guangdong Ocean Univ, Coll Food Sci & Technol, Sch Chem & Environm, 518114, Zhanjiang 524088, Shenzhen, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab, Zhanjiang 524025, Peoples R China;[3]Jeju Natl Univ, Dept Marine Life Sci, Jeju City, South Korea

年份：2022

卷号：92

外文期刊名：JOURNAL OF FUNCTIONAL FOODS

收录：SCI-EXPANDED(收录号：WOS:000794211300004)、、Scopus(收录号：2-s2.0-85128162027)、WOS

基金：The research was funded by the 2020 Shenzhen International Scientific and Technological Cooperation R&D Project (GJHZ20190823111601682) and the Natural Science Foundation of Guangdong Province (2020A1515011075). The supported by the Development Project about Marine Economy Demonstration of Zhanjiang City (XM-202008-01B1) and Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang, ZJW-2019-07).

语种：英文

外文关键词：Isochrysis Zhanjiangensis; Peptide; Hypertension; Angiotensin-I Converting Enzyme; Inflammation; Apoptosis

外文摘要：Microalgae are primary producers in marine ecosystems, and have been diffusely used in medicine and food. In this research, we evaluated the protective effects of polypeptides from microalgae, Isochrysis zhanjiangensis (EMFGTSSET, ETT) on endothelial injury in angiotensin II (Ang II)-induced human umbilical vein endothelial cells (HUVEC). The results showed that ETT inhibited the fluorescence intensity of intracellular reactive oxygen species (ROS), and reduces the expression of related cytokines, such as IL-1(i, IL-8, TNF-alpha, iNOS, COX-2, ET-1, AT-1, and adhesion factor. It blocks inflammation and apoptosis through mitogen-activated protein kinases (MAPK), nuclear factor kappa B (NF-kappa B) and serine/threonine kinase (Akt) signal pathways, thereby alleviating endothelial damage. Meanwhile, ETT (IC50 = 15.08 mu M) antagonizes Angiotensin-I Converting Enzyme (ACE) in a non-competitive binding mode, and has an antihypertensive effect in spontaneously hypertensive rats (SHR). Therefore, ETT has excellent effect in regulating hypertension, can become the main component of potential functional food for the prevention of cardiovascular diseases, and further improve the use value of Isochrysis Zhanjiangensis.