文献类型：期刊文献

英文题名：Microbiota Diversity in Pearl Oyster Pinctada fucata martensii Intestine and Its Aquaculture Environment

作者：Zheng, Zhe[1,2,3,4];Liao, Yongshan[1,2];Ye, Jianming[1];Yang, Chuangye[1,2,3,4];Adzigbli, Linda[1,5];Wang, Qingheng[1,2,3,4];Du, Xiaodong[1,2,3,4];Deng, Yuewen[1,2,3,4]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Peoples R China;[2]Pearl Breeding & Proc Engn Technol Res Ctr Guangd, Zhanjiang, Peoples R China;[3]Guangdong Prov Engn Lab Mariculture Organism Bree, Zhanjiang, Peoples R China;[4]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[5]Univ Rostock, Dept Marine Biol, Rostock, Germany

年份：2021

卷号：8

外文期刊名：FRONTIERS IN MARINE SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000637010400001)、、Scopus(收录号：2-s2.0-85103858025)、WOS

基金：This work was supported by the Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams, Department of Agriculture and Rurual Affairs of Guangdong Province (Grant no. 2020KJ146), Young Innovative Talents Project (Grant no. 2019KQNCX043), Innovation Team Project (Grant no. 2017KCXTD016) from the Department of Education of Guangdong Province, Guangdong Basic and Applied Basic Research Foundation (Grant nos. 2019A1515111026 and 2020A1515010691), the China Agriculture Research System (Grant no. CARS-049), and Students Innovation and Entrepreneurship Training Program (Grant no. CXXL2020045).

语种：英文

外文关键词：Pinctada fucata martensii; bacterial community; intestine; environmental microbiota; 16S rRNA-based sequencing

外文摘要：Environmental microbiota plays a vital role in the intestinal microbiota of aquatic organisms. However, data concerning the association between the intestinal microbiota of pearl oyster Pinctada fucata martensii and the surrounding seawater are limited. The existing bacterial communities in pearl oyster intestine and surrounding water from two sites (D and H, within Liusha Bay in Guangdong, China) were investigated using 16S rRNA-based sequencing to explore the relationship among the two. D located in the inner bay, and H located in the open sea area outside bay. Results revealed the richness and diversity of pearl oyster intestinal microbiota to be less than those of the surrounding water, with 38 phyla and 272 genera observed as a result of the classifiable sequence. The microbiota compositions in the intestine and the surrounding water were diversified at the phylum and genus levels, with the sequencing data being statistically significant. However, the functional prediction of microbiota emphasized the overall similarity in the functional profile of the surrounding seawater and intestinal microbiomes. This profile was associated with metabolism of cofactors and vitamin, carbohydrates metabolism, amino acids metabolism, metabolism of terpenoids, and polyketides, metabolism of other amino acids, lipids metabolism, and energy metabolism. Seven common operational taxonomic units (OTUs), which belonged to phyla Tenericutes, Cyanobacteria, and Planctomycetes, were noted in the intestines of pearl oysters from two different sites. These OTUs may be affiliates to the core microbiome of pearl oyster. Significantly different bacterial taxa in the intestines of pearl oysters from two different sites were found at the phylum and genus levels. This finding suggested that the bacterial communities in pearl oyster intestines may exhibit some plasticity to adapt to changes in the surrounding water-cultured environment. This study generally offers constructive discoveries associated with pearl oyster intestinal microbiota and provides guidance for sustainable aquaculture.