文献类型：期刊文献

中文题名：T-2毒素对凡纳滨对虾DNA黏度损伤效应

英文题名：Effect of T-2 Toxin on Viscosity of DNA from Litopenaeus vannamei

作者：宁守强[1];王雅玲[1];王小博[1];邱妹[1];孙力军[1];郭俊豪[1];潘晓梅[1];励建荣[2]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东普通高等学校水产品深加工重点实验室,广东湛江524088;[2]渤海大学辽宁省食品安全重点实验室,辽宁锦州121013

年份：2018

卷号：39

期号：5

起止页码：173

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD、、CSCD_E2017_2018、EI(收录号：20182205253587)、北大核心2017、Scopus、北大核心、CSCD

基金：国家自然科学基金面上项目(31171634;31371777)

语种：中文

中文关键词：T-2毒素;响应面分析;基因组DNA;黏度分析

外文关键词：T-2 toxin; response surface methodology; genomic DNA; viscosity analysis

中文摘要：为探究T-2毒素对凡纳滨对虾DNA黏度的损伤效应,通过小牛胸腺DNA与T-2毒素作用,采用响应面分析T-2毒素与DNA的最佳作用条件,通过体外T-2毒素与对虾DNA作用验证,以及喂食对虾T-2毒素进行蓄积染毒后,观察其对DNA黏度的影响。结果:响应面分析优化出对DNA黏度影响最明显的反应条件为T-2毒素质量浓度2.70 ng/mL、DNA质量浓度50μg/mL、反应时间43 min;T-2毒素与对虾DNA体外作用,随着T-2毒素质量浓度的增加,DNA黏度随之增大,在T-2毒素质量浓度为2.70 ng/mL时达到最大值,此后降低;喂食对虾T-2毒素进行蓄积染毒对对虾DNA黏度的影响无明确规律,与体外对虾DNA实验结果并不相似。本研究阐明T-2毒素对对虾DNA黏度的影响,对探讨T-2毒素的毒理作用具有一定的意义。

外文摘要：The objective of this paper was to explore the effect of T-2toxin on the viscosity of DNA from shrimps(Litopenaeus vannamei).The conditions for the reaction between calf thymus DNA and T-2toxin were optimized using response surface methodology.The reaction between shrimp DNA and T-2toxin in vitro was investigated.Furthermore,the effect of T-2toxin on DNA viscosity was examined after feeding shrimps with T-2toxin for20days to cause accumulative poisoning.The results obtained showed that a T-2toxin concentration of2.70ng/mL,a DNA concentration of50μg/mL and a reaction time of43min were determined to be the optimum conditions for the greatest effect of T-2toxin on DNA viscosity.DNA viscosity rose with increasing concentration of T-2toxin,reaching its maximum level at2.70ng/mL,followed by a decrease.The effect of T-2toxin accumulation in shrimps on the viscosity of DNA was not clear.This result was dissimilar to the result in vitro.In conclusion,this study may be of great significance for understanding the toxicological effect of T-2toxin on shrimps.