文献类型：期刊文献

英文题名：Ultrasound-mediated modification of biological properties of Sepia esculenta ink polysaccharide SIP4

作者：Wu, Jiayi[1];Lin, Zhihan[1];Wang, Xiaolu[1];Huang, Yiling[1];Luo, Ping[1];Liu, Huazhong[1]

机构：[1]Guangdong Ocean Univ, Sch Chem & Environm, Dept Appl Chem, Zhanjiang 524088, Peoples R China

年份：2026

卷号：380

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:001854504300001)、、EI(收录号：20263421338791)、Scopus(收录号：2-s2.0-105047818218)、WOS

基金：This work was predominantly funded by the Guangdong Basic and Applied Basic Research Foundation (No. 2022A1515012091) , and the Special Project in Key Fields of Guangdong Universities (No. 2022ZDZX2025) .

语种：英文

外文关键词：SIP4; Ultrasound degradation; Biological properties

外文摘要：Ultrasonication is increasingly used as an eco-friendly method to depolymerize natural polysaccharides for improving physicochemical properties and bioactivities of the native macromolecules. However, a systematic evaluation is required to determine how degradation-induced alterations in polysaccharide molecular properties modulate their biological functions. To this end, this study focuses on SIP4, a bioactive sulfated glycosaminoglycan from Sepia esculenta ink, to clarify the physicochemical changes caused by ultrasonic depolymerization and evaluate their subsequent impact on biological activities. This work treated SIP4 using ultrasound at 50 degrees C, 450 W for 90 min. Physicochemical and biological properties of SIP4 and its degradation products were evaluated. Data indicated that ultrasonic treatment successfully depolymerized SIP4, the weight-average molecular weight (Mw) declined from 24.96 to 14.27 kDa, at the duration of 90 min. The depolymerization process fits a second-order kinetic model. The degradation did not modify the infrared spectral characteristics, but elevated the sulfate group content of the carbohydrate. Interestingly, depolymerized products showed procoagulant activity, which was opposite to SIP4 with anticoagulation. Additionally, ultrasound treatment modified some biological properties of SIP4, such as repressing the activation of alpha-glucosidase and tyrosinase, improving the bile salt binding capacity, and altering antioxidation demonstrated by increased scavenging activity for hydroxyl radical and decreased scavenging ability for DPPH and superoxide anion radicals. These data suggest that controlled ultrasonication is effective for modifying the biological properties of SIP4, which can effectively adapt SIP4 for specific practical uses, including hemostatic agents and functional foods for lowering blood lipid.