文献类型：期刊文献

中文题名：配合饲料替代冰鲜杂鱼对高体鰤幼鱼消化道结构功能、免疫及抗氧化能力的影响

英文题名：Formulated feed induced structural,functional,immunity-related,and antioxidant capacity changes in the digestive tracts of juvenile greater amberjack(Seriola dumerili)

作者：李锦[1];黄洋[1,2];茹笑影[2];陈培培[1];杨佳慧[1];杨桐林[1];朱琨锋[1];邹一帆[1];朱春华[1,2]

机构：[1]广东海洋大学水产学院,广东省名特优鱼类生殖调控与繁育工程技术研究中心,广东省海水养殖生物育种工程实验室,广东湛江524088;[2]南方海洋科学与工程广东省实验室,广东湛江524025

年份：2025

卷号：49

期号：6

起止页码：48

中文期刊名：海洋科学

外文期刊名：Marine Sciences

收录：北大核心2023、、北大核心

基金：广东海洋大学2023年校级第一批“揭榜挂帅”项目(030301022301);海南省重大科技计划项目(ZDKJ2021011);高体鰤规模化早繁技术研究与人工颗粒饲料高效养殖示范(ZJW-2023010)。

语种：中文

中文关键词：高体鰤;饲料替代;酶活;组织结构;肠道菌群;免疫相关基因

外文关键词：Seriola dumerili;feed substitution;digestive tract index;digestive enzymes;serum biochemical indicators;antioxidant enzymes;immune-related genes

中文摘要：通过60 d的养殖实验,研究了配合饲料替代冰鲜杂鱼对高体鰤(Seriola dumerili)幼鱼消化道指数及消化酶活性、肠道组织结构及菌群多样性、血清生化指标、肝脏免疫相关基因表达以及抗氧化酶活性的影响。结果表明:相较于冰鲜杂鱼组,投喂配合饲料组高体鰤幼鱼胃体比显著降低,而肠体比、肠道胰蛋白酶活性和肠道绒毛高度显著增加,表明配合饲料导致高体鰤幼鱼肠道消化能力减弱并且导致致病菌的丰度增加以至肠道健康状况下滑。此外,配合饲料投喂降低了血清中高密度脂蛋白(HDL-C)、甘油三酯(TG)和总胆固醇(TC)的含量,但通过调控MyD88的信号通路改善了免疫功能。另外,配合饲料通过加强血清中总抗氧化能力(T-AOC),降低血清和肝脏中丙二醛(MDA)的含量以增强其抗氧化能力。研究证明开发高体鰤幼鱼配合饲料具有很大的潜力,但后续需要进一步调整和优化饲料配方。

外文摘要：The effects of replacing chilled trash fish with formulated feed on the digestive tract index and digestive enzyme activities,intestinal tissue structure and bacterial diversity,serum biochemical indices,hepatic immune-related gene expression,and antioxidant enzymes in the juvenile greater amberjack(Seriola dumerili)were studied in a 60-day aquaculture experiment.The stomach/body weight of juveniles was significantly lower in the formulated feed group compared to the chilled trash fish group.In contrast,the intestinal/body weight,intestinal trypsin activity,and villi length were markedly greater.These results indicated that the formulated feed reduced digestive capacity in the intestines of the juveniles and also enhanced the abundance of pathogenic bacteria,leading to a decline in intestinal health.Additionally,it suppressed the serum levels of high-density lipoproteins,triglycerides,and total cholesterol but improved the immune function by regulating the MyD88 signaling pathway.It also enhanced the serum total antioxidant capacity but reduced the serum and liver malondialdehyde levels,thereby bolstering the antioxidant capacity.Thus,this study demonstrated the excellent potential of developing a compound feed for juvenile greater amberjack while noting that the formulated feed should be further adjusted and optimized through further research.