文献类型：期刊文献

英文题名：MicroRNA, Pm-miR-2305, Participates in Nacre Formation by Targeting Pearlin in Pearl Oyster Pinctada martensii

作者：Jiao, Yu[1];Zheng, Zhe[1];Tian, Rongrong[1];Du, Xiaodong[1,2];Wang, Qingheng[1];Huang, Ronglian[1]

机构：[1]Guangdong Ocean Univ, Fishery Coll, Zhanjiang 524025, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Guangdong Technol Res Ctr Pearl Aquaculutre & Pro, Zhanjiang 524025, Peoples R China

年份：2015

卷号：16

期号：9

起止页码：21442

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：SCI-EXPANDED(收录号：WOS:000364541000073)、、WOS

基金：The studies were financially supported by grants of the National Natural Science Foundation of China (41206141, 31272635 and 31372526), Natural Foundation of Guangdong Ocean University (1212318), Excellent Young Scientist Foundation of Guangdong Ocean University (201402).

语种：英文

外文关键词：Pinctada martensii; biomineralization; nacre formation; miR-2305

外文摘要：MicroRNAs (miRNAs) are noncoding RNA molecules that function as negative regulators of target genes. In our previous research, 258 pm-miRNAs were identified in Pinctada martensii by Solexa deep sequencing. Pm-miR-2305 was one of the identified pm-miRNAs with a potential function in biomineralization. In the present study, the precursor of pm-miR-2305 was predicted with 96 bp, containing a characteristic hairpin structure. Stem-loop qRT-PCR analysis indicated that pm-miR-2305 was constitutively expressed in all the tissues (adductor muscle, gill, mantle, hepatopancreas, foot, and gonad) of P. martensii and was highly expressed in the foot. After the over-expression of pm-miR-2305 in the mantle by mimics injection into the muscle of P. martensii, nacre demonstrated disorderly growth, as detected by scanning electron microscopy. Dual luciferase reporter gene assay indicated that pm-miR-2305 mimics could significantly inhibit the luciferase activity of the reporter containing the 3UTR of the pearlin gene. Western blot analysis demonstrated that the protein expression of pearlin was down-regulated in the mantle tissue after the over-expression of pm-miR-2305. Therefore, our data showed that pm-miR-2305 participated in nacre formation by targeting pearlin in P. martensii.