文献类型：期刊文献

中文题名：岩藻多糖对断奶羔羊小肠组织形态、消化酶活性和抗氧化指标的影响

英文题名：Effects of Fucoidan on Small Intestinal Tissue Morphology,Digestive Enzyme Activity and Antioxidant Indices of Weaned Lambs

作者：郭广振[1];陈佳怡[1];彭苏[1];邱晓媛[1];牛志力[1];吴卓明[1];兰瑞霞[1];高振华[1];赵志辉[1];尹福泉[1]

机构：[1]广东海洋大学滨海农业学院,湛江524088

年份：2022

卷号：34

期号：8

起止页码：5206

中文期刊名：动物营养学报

外文期刊名：CHINESE JOURNAL OF ANIMAL NUTRITION

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：广东省农业农村厅-广东省现代草牧业(羊)产业技术体系项目(2019KJ127);2019年广东省科技厅项目-雷州山羊健康养殖技术集成(K19015);广东省科技专项资金(“大专项”+“任务清单”)项目-雷州山羊保育繁推一体化生态健康养殖关键技术创新与示范;华南农业大学-广东海洋大学高校服务乡村振兴共同体黑山羊养殖协同服务;2020年国家级大创-宏组学分析岩藻多糖介导瘤胃微生物对雷州山羊免疫调控机理研究(580520011);广东海洋大学2022年社会服务专项资金项目-遂溪县城月镇肉羊健康养殖技术示范与推广。

语种：中文

中文关键词：岩藻多糖;早期断奶羔羊;肠道通透性;消化酶活性;抗氧化

外文关键词：fucoidan;early-weaned lambs;intestinal permeability;digestive enzyme activity;antioxidant

中文摘要：本试验旨在探讨代乳粉添加岩藻多糖对断奶羔羊小肠通透性、组织形态、消化酶活性及抗氧化指标的影响。选择24只平均体重为(7.32±0.37) kg的30日龄健康川中黑山羊公羔,随机分为4个组,每组6个重复,每个重复1只羊。Ⅰ组(对照组)饲喂不含岩藻多糖的代乳粉,Ⅱ组、Ⅲ组和Ⅳ组分别在代乳粉中添加0.1%、0.3%和0.6%的岩藻多糖。试验预试期7 d,正试期30 d。结果表明:1)Ⅲ组和Ⅳ组羔羊血清二胺氧化酶活性和D-乳酸含量显著低于Ⅰ组(P<0.05);各组间血清内毒素含量差异不显著(P>0.05)。2)Ⅲ组羔羊小肠各肠段绒毛高度、绒毛高度/隐窝深度(V/C)以及肌层和黏膜厚度显著高于Ⅰ组(P<0.05);各组小肠隐窝深度之间无显著差异(P>0.05)。3)Ⅲ组和Ⅳ组羔羊空肠α-淀粉酶和乳糖酶活性显著高于Ⅰ组(P<0.05);各组间空肠脂肪酶、胰蛋白酶和糜蛋白酶活性差异不显著(P>0.05)。4)随着代乳粉中岩藻多糖添加水平的提高,羔羊小肠抗氧化酶活性呈先升后降趋势,小肠丙二醛(MDA)含量则呈先降后升趋势。Ⅲ组和Ⅳ组羔羊十二指肠、空肠总抗氧化能力(T-AOC)、过氧化氢酶(CAT)活性显著高于Ⅰ组(P<0.05);Ⅱ组空肠T-AOC、CAT活性显著高于Ⅰ组(P<0.05);Ⅲ组十二指肠、空肠谷胱甘肽过氧化物酶(GSH-Px)活性以及Ⅲ组和Ⅳ组空肠超氧化物歧化酶(SOD)活性显著高于Ⅰ组(P<0.05);岩藻多糖添加组小肠各肠段(Ⅱ组回肠除外) MDA含量显著低于Ⅰ组(P<0.05)。综上所述,代乳粉中添加岩藻多糖能有效降低断奶羔羊血清D-乳酸含量和二胺氧化酶活性,提高小肠黏膜厚度、肌层厚度、绒毛高度及V/C,并提高空肠α-淀粉酶和乳糖酶活性以及小肠抗氧化能力,改善小肠肠道屏障结构与功能,从而在一定程度上提高断奶羔羊的肠道健康;在本试验条件下,代乳粉中岩藻多糖的适宜添加水平为0.3%。

外文摘要：This experiment was conducted to determine the effects of fucoidan supplementation in milk replacer on intestinal permeability,tissue morphology,digestive enzyme activity and antioxidant indices of weaned lambs.Twenty-four healthy 30-day-old Chuanzhong black male lambs with similar body weight of(7.32±0.37)kg were randomly divided into 4 groups with 6 replicates per group and 1 lamb per replicate.Lambs in groupⅠ(control group)were fed the milk replacer without fucoidan,and the others in groupⅡ,groupⅢand groupⅣwere fed the milk replacer supplemented with 0.1%,0.3%and 0.6%fucoidan,respectively.The pre-experimental period lasted for 7 days,and the experimental period lasted for 30 days.The results showed as follows:1)the serum diamine oxidase activity and D-lactic acid content of lambs in groupⅢand groupⅣwere significantly lower than those in groupⅠ(P<0.05),and there was no significant difference in serum endotoxin content among all groups(P>0.05).2)The villus height,ratio of villus height to crypt depth(V/C),and thickness of muscularis and mucosa in segments of the small intestine of lambs in groupⅢwere significantly higher than those in groupⅠ(P<0.05),and there was no significant difference in crypt depth of small intestine among all groups(P>0.05).3)The activity ofα-amylase and lactase in jejunum of lambs in groupⅢand groupⅣwas significantly higher than that in groupⅠ(P<0.05),and there was no significant difference in activity of lipase,trypsin and chymotrypsin in jejunum among all groups(P>0.05).4)With the increasing of fucoidan supplemental level in milk replacer,the antioxidant enzyme activity in small intestine of lambs firstly increased and then decreased,and the malonaldehyde(MDA)content in small intestine firstly decreased and then increased.In addition,the total antioxidant capacity(T-AOC)and catalase(CAT)activity in duodenum and jejunum of lambs in groupⅢand groupⅣwere significantly higher than those in groupⅠ(P<0.05),the T-AOC and CAT activity in jejunum in groupⅡwere significantly higher than those in groupⅠ(P<0.05),the glutathione peroxidase(GSH-Px)activity in duodenum and jejunum in groupⅢand the superoxide dismutase(SOD)activity in jejunum in groupⅢand groupⅣwere significantly higher than those in groupⅠ(P<0.05),and the MDA content in segments of the small intestine in fucoidan supplemental groups(except for the ileum in groupⅡ)was significantly higher than that in groupⅠ(P<0.05).In conclusion,the fucoidan supplementation in milk replacer can effectively decrease the serum D-lactic acid content and diamine oxidase activity of lambs,increase the small intestine mucosa thickness,muscularis thickness,villus height and V/C,enhance the activity ofα-amylase and lactase in jejunum and small intestinal antioxidant capacity,and improve the small intestinal barrier structure and function,thus improving the intestinal health of weaned lambs;under the experimental condition,the suitable supplemental level of fucoidan in milk replacer is0.3%.