文献类型：期刊文献

英文题名：Ultrasensitive detection of microRNA using an array of Au nanowires deposited within the channels of a porous anodized alumina membrane

作者：Li, Chengyong[1,2];He, Lei[1];Jiang, Shiqi[1,2];Mo, Rijian[1];Yan, Xiemin[1];Qian, Zhong-Ji[1,2];Zhou, Chunxia[1];Sun, Shengli[2];Hong, Pengzhi[1];Huang, Yujun[3]

机构：[1]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Peoples R China;[2]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[3]Potou Dist Peoples Hosp, Dept Orthoped, Zhanjiang 524003, Peoples R China

年份：2019

卷号：102

起止页码：19

外文期刊名：ELECTROCHEMISTRY COMMUNICATIONS

收录：SCI-EXPANDED(收录号：WOS:000465448100004)、、EI(收录号：20191306706563)、Scopus(收录号：2-s2.0-85063526299)、WOS

基金：This work is supported by the National Natural Science Foundation of China (21874029), Guangdong Yangfan Program (201635018), Guangdong Special Support Program (2017TQ04N706), Science and Technology Planning Project of Shenzhen City (JCYJ20170818111719650) and Industrial Development Special Funds of Dapeng New Area (KY20170209, KY20180202).

语种：英文

外文关键词：Nanopores; Label-free detection; microRNA; Porous anodic alumina

外文摘要：A new nanopore array/Au electrode was fabricated and applied to the detection of microRNA. The electrode was prepared via alternating current electrodeposition of Au nanowires in the nanopores of a porous anodic alumina membrane (without the need to remove aluminum or any barrier layer). The internal surface of the nanopores was then functionalized with an electrically neutral morpholino with a specially designed sequence. The resulting electrode shows excellent sensitivity and selectivity in detection of the target miRNA-155. The detection limit is 10 aM and the linear range 10 aM-1 nM in aqueous solution and the electrode can be reused in 6M urea solution. In addition, it shows good linearity (100 aM-0.1 nM) in a serum sample. This new biosensor is expected to enhance miRNA detection in clinical diagnosis.