文献类型：期刊文献

中文题名：姜黄素纳米硒的制备工艺优化、表征及其对酒精性肝损伤的保护作用

英文题名：Preparation Process Optimization and Characterization of Selenium-Curcumin Nanoparticles and Its Protective Effect on Alcoholic Liver Injury

作者：黄文浩[1];陈建平[1,2];陈博文[1];陈岑[1];钟赛意[1,2];刘晓菲[1,2];李瑞[1,2];宋兵兵[1,2];汪卓[1,2]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,水产品深加工广东普通高等学校重点实验室,广东省亚热带果蔬加工科技创新中心,广东湛江524088;[2]大连工业大学,海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034

年份：2024

卷号：45

期号：5

起止页码：100

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：北大核心2023、CSTPCD、、EI(收录号：20240915649522)、Scopus、北大核心

基金：广东省自然科学基金面上项目(2020A1515010860,2021A1515012455);广东海洋大学创新强校项目(230419100);广东海洋大学“南海学者计划”项目(002029002009)。

语种：中文

中文关键词：姜黄素纳米硒;制备工艺;响应面优化;结构表征;酒精性肝损伤

外文关键词：selenium-curcumin nanoparticles;preparation process;response surface optimization;structural characterization;alcoholic liver injury

中文摘要：目的:探索姜黄素纳米硒(Selenium-curcumin nanoparticles,SeNPs@Cur)的最佳制备工艺参数,并评价其对酒精性肝损伤的保护作用。方法:在单因素实验基础上,以粒径为指标,选择姜黄素浓度、抗坏血酸与亚硒酸钠摩尔比(V_(C):Na_(2)SeO_(3))、反应时间和反应温度为考察因素,通过响应面优化SeNPs@Cur的工艺参数,并采用扫描电子显微镜(Scanning electron microscope,SEM)、傅里叶变换红外光谱仪(Fourier transform infrared spectrometer,FT-IR)和能谱仪(Energy dispersive spectrometer,EDS)表征其结构。采用MTT法测定其对乙醇诱导LO2细胞损伤的保护作用。结果:SeNPs@Cur的最佳制备工艺参数为:姜黄素浓度5.92 mg/mL、V_(C):Na_(2)SeO_(3)摩尔比10.39:1、反应时间1.98 h和反应温度34.00℃,在此条件下制备得到的SeNPs@Cur粒径为(100.79±3.46)nm,样品中姜黄素的载药率为12.80%±0.80%,硒含量为23.32%±0.07%。进一步的结构鉴定发现,制备所得的SeNPs@Cur为分散的球形纳米粒。而且,与模型组相比,经不同浓度SeNPs@Cur处理后,细胞活力从58.06%±0.43%分别提高至71.43%±1.39%、77.33%±3.54%和85.41%±4.61%,表明SeNPs@Cur对乙醇损伤LO2细胞具有保护作用,且呈浓度依赖性。结论:成功获得了具有抗酒精性肝损伤作用的SeNPs@Cur,为姜黄素的剂型改善及其应用提供了新的技术手段和理论参考数据。

外文摘要：Objective:In this work,the optimal preparation process parameters of selenium-curcumin nanoparticles(SeNPs@Cur)were explored and its protective effect on alcoholic liver injury was evaluated.Methods:On the basis of single factor experiment,with particle size as the index,curcumin dosage,molar ratio of V_(C)to Na_(2)SeO_(3),reaction time and reaction temperature were selected as influence factors.The process parameters of SeNPs@Cur were optimized by response surface methodology,and its structure was characterized by scanning electron microscope(SEM),fourier transform infrared spectrometer(FT-IR)and energy dispersive spectrometer(EDS).The protective effect of SeNPs@Cur on ethanol-induced LO2 cell injury was investigated by MTT method.Results:The optimal conditions of SeNPs@Cur were list as follows:Curcumin dosage of 5.92 mg/mL,molar ratio of V_(C)to Na_(2)SeO_(3)of 10.39:1,the reaction time of 1.98 h,and the reaction temperature of 34.00℃.Under these conditions,the SeNPs@Cur with a minimum size of(100.79±3.46)nm was obtained.The loading rate of curcumin and the selenium content in the sample were 12.80%±0.80%and 23.32%±0.07%,respectively.Further structural identification showed that the prepared SeNPs@Cur was dispersed spherical nanoparticles.Moreover,compared with the model group,after treatments with different dosages of SeNPs@Cur,the cell viabilities increased from 58.06%±0.43%to 71.43%±1.39%,77.33%±3.54%,and 85.41%±4.61%,respectively,indicating that SeNPs@Cur had a protective effect on LO2 cells damaged by ethanol in a concentration-dependent manner.Conclusion:The SeNPs@Cur with anti-alcoholic liver injury effect was successfully obtained,which provided new technical means and theoretical reference data for formulation improvement and application of curcumin.