文献类型：期刊文献

中文题名：鱼鳔糖胺聚糖的提取及其吸湿保湿性能评价

英文题名：Extraction of glycosaminoglycans from swim bladder and evaluation of the capacities of its hygroscopicity and moisture retention

作者：屈义[1];周斯仪[1];冯陶[1];钟赛意[1,2];谌素华[1];苏伟明[1];吴小禾[2,3]

机构：[1]广东海洋大学食品科技学院广东省水产品加工与安全重点实验室广东省海洋食品工程技术研究中心水产品深加工广东省高等学校重点实验室,广东湛江524088;[2]中山市淡水产品技术服务协同创新中心,广东中山528436;[3]中山火炬职业技术学院,广东中山528436

年份：2017

卷号：38

期号：16

起止页码：118

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD、、北大核心2014、CSCD_E2017_2018、北大核心、CSCD

基金：广东省高等教育"创新强校工程"专项资金(GDOU2016050203);2017年广东省海洋和渔业发展专项资金生物医药与功能性制品研发2;中山市淡水产品技术服务协同创新中心项目(2016C1007)

语种：中文

中文关键词：鱼鳔;糖胺聚糖;提取工艺;理化及结构特征;吸湿性;保湿性

外文关键词：swim bladder; glycosaminoglycan; extraction process; physic-chemical characters and structure properties; hygroscopicity; moisture retention;

中文摘要：以鱼鳔为原料,通过枯草中性蛋白酶和胰蛋白酶双酶酶解提取糖胺聚糖,在单因素实验的基础上采用响应曲面法对提取条件进行优化,通过理化分析及UV、IR分析其性质和化学结构特征,并评价其吸湿保湿性。结果表明:酶法提取鱼鳔糖胺聚糖的最佳工艺条件:料液比1∶20 g∶m L、酶解时间4 h,酶解温度50℃,酶用量7%,酶解p H8,最优条件下鱼鳔糖胺聚糖粗品得率为1.19%,糖胺聚糖含量为19.09%;红外吸收光谱检测表明,其具有典型的糖胺聚糖特征吸收峰,且含有α糖苷键的吡喃糖环结构;鱼鳔糖胺聚糖具有良好的吸湿保湿性,总体吸湿保湿性优于壳聚糖、海藻酸钠等常规保湿剂。综上表明,鱼鳔糖胺聚糖作为一种天然的保湿剂具有一定的应用前景。

外文摘要：The extraction process of glycosaminoglycan from swim bladder via enzymatic hydrolysis by pancreatin and neutral protease of Bacillus subtilis were optimized by response surface methodology based on single factor experiments,the physic-chemical characters and structure properties were analyzed by UV and IR spectroscopy,and the evaluation of the capacities of hygroscopicity and moisture retention were conducted in this investigation. The results showed that the optimum processing conditions of enzyme hydrolysis was solid-liquid ration of 1 ∶ 20 g ∶ m L,enzymolysis time of 4 h,enzymolysis temperature of50 ℃,enzyme dosage of 7%,and the enzmolysis p H of 8.0. Under these optimum conditions,the yield and content of glycosaminoglycan were of 1.19%,19.09%.Moreover,the IR spectrum showed that the extracted glycosaminoglycan had typical characteristic absroption peaks of polysaccride,and comprised a pyranose ring structure having an α glycosidic bond.Finally,the extracted glycosaminoglycan had excellent capacities of hygroscopicity and moisture retention,which were superior to those of the conventional humectants,such as chitosan and sodium alginate. These results clearly established the possibility that swim bladder glycosaminoglycan could be effectively employed as a moisturizer.