文献类型：期刊文献

英文题名：miR-204 Regulates the Proliferation of Dairy Goat Spermatogonial Stem Cells via Targeting to Sirt1

作者：Niu, Bowen[1];Wu, Jiang[1,2];Mu, Hailong[1];Li, Bo[1];Wu, Chongyang[1];He, Xin[1];Bai, Chunling[3];Li, Guangpeng[3];Hua, Jinlian[1]

机构：[1]Northwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Key Lab Anim Biotechnol Agr,Minist China, Yangling 712100, Shaanxi, Peoples R China;[2]Guangdong Ocean Univ, Coll Agr, Zhanjiang, Peoples R China;[3]Inner Mongolia Univ, Key Lab Mammalian Reprod Biol & Biotechnol, Minist Educ, Hohhot, Peoples R China

年份：2016

卷号：19

期号：2

起止页码：120

外文期刊名：REJUVENATION RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000374649800004)、、Scopus(收录号：2-s2.0-84964727017)、WOS

基金：This work was supported by the National Natural Science Foundation of China (grant no. 31272518, 31572399), the National Major Project for the Production of Transgenic Breeding (grant no. 2014ZX08007002-010), the National High Technology Research and Development Program of China (grant no. SS2014AA021605), the Doctoral Fund of the Ministry of Education of PR China (RFDP, grant no. 20120204110030), and the Program of the Shaanxi Province (2015NY157).

语种：英文

外文摘要：The regulation of spermatogonial stem cell (SSC) proliferation and self-renewal is a complex process. Several studies on the microRNA regulation of mammalian spermatogenesis have been reported. Here, we predicted miRNA targeting of Sirt1, and a dual luciferase experiment confirmed that miR-204 interacted with the Sirt1 3-untranslated region (3-UTR). The expression of miR-204 and Sirt1 in dairy goat testicles was investigated, and the results showed that the expression pattern of Sirt1 was similar to that of miR-204 in the temporal-spatial distribution. The over-expression of Sirt1 in goat SSCs can promote SSCs' self-renewal gene expression and cell proliferation. Furthermore, miRNA sequencing results showed that Sirt1 had a higher expression level in dairy goat CD49f(+) and CD90(+) SSCs, but the expression level of miR-204 was lower. In an in vitro assay, Sirt1 was significantly down-regulated in dairy goat SSCs when transfected with miR-204 mimics, indicating that Sirt1 was a target of miR-204 in the dairy goat. On the basis of the results of RT-qPCR, fluorescence-activated cell sorting (FACS), and western blotting, we found that the over-expression of Sirt1 in goat SSCs can promote cellular proliferation and change self-renewal and pluripotent gene expression. Thus, miR-204 was involved in the regulation of dairy goat SSCs proliferation via Sirt1.