文献类型：期刊文献

中文题名：2株水产病原气单胞菌拮抗菌的筛选鉴定及生物学特性

英文题名：Screening,Identification and Biological Characteristics of Two Antagonistic Bacteria Against Aquaculture Aeromonas Pathogens

作者：龙梦[1,2,3];樊慧敏[1];江遥[3];夏洪丽[1];程俊[1];喻大鹏[1];夏立群[1,4];鲁义善[1,4]

机构：[1]广东海洋大学深圳研究院,广东省水生动物健康评估工程技术研究中心,深圳市海水经济动物种苗健康评价公共技术服务平台,广东深圳518120;[2]深圳市大鹏新区科技创新服务中心,广东深圳518120;[3]中国科学院水生生物研究所,湖北武汉430072;[4]广东海洋大学水产学院,广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524088

年份：2022

卷号：41

期号：6

起止页码：927

中文期刊名：水产科学

外文期刊名：Fisheries Science

收录：CSTPCD、、北大核心、CSCD、北大核心2020、CSCD_E2021_2022

基金：国家重点研发计划项目(2020YFD0900201);广东省农业产业体系项目(2019KJ141);广东省重点领域研发计划项目(2020B0202010009);深圳市科技计划项目(JCYJ20180507183240459);深圳市大鹏新区产业发展专项资金资助项目(PT202001-12);中国博士后科学基金资助项目(2019M662750).

语种：中文

中文关键词：气单胞菌;出血性败血症;贝莱斯芽孢杆菌;解淀粉芽孢杆菌;拮抗活性;益生菌

外文关键词：Aeromonas;haemorrhagic septicaemia;Bacillus velezensis;B.amyloliquefaciens;antagonistic activity;probiotics

中文摘要：气单胞菌是水产养殖鱼类的重要病原菌,暂无针对多种气单胞菌感染防治的有效措施。红树林沉积物中芽孢杆菌丰富,是活性微生物的重要储库。笔者自深圳东涌红树林沉积物中筛选获得2株对6种鱼类病原性气单胞菌均具有较强拮抗活性的菌株AH10和AQ1,基于形态学、生理生化特性和分子生物学分析结果,将2株拮抗菌鉴定为解淀粉芽孢杆菌和贝莱斯芽孢杆菌。生物学特性测试结果表明,2株菌的生长特性相似,最适生长温度为32℃、pH 5~7、盐度0~20。药敏试验结果显示,2株菌除对四环素耐药、对链霉素中度敏感外,对绝大多数抗生素均高度敏感。拮抗试验结果显示,2株菌除对嗜水气单胞菌、舒氏气单胞菌等6种气单胞菌具有拮抗作用外,亦均对爱德华氏菌、类志贺邻单胞菌等水产病原菌具有拮抗作用。其中,解淀粉芽孢杆菌AH10和贝莱斯芽孢杆菌AQ1分别对类志贺邻单胞菌和杀鲑气单胞菌的拮抗作用最强。生物安全试验表明,2株菌对斑马鱼和鲢均无致病性,生物安全性良好。本研究结果可为水产养殖中气单胞菌病的生物防治提供新的微生物资源,具有较好的应用前景和进一步研究价值。

外文摘要：Aeromonas is an important pathogen of aquaculture,and there are still no effective treatments to prevent the infections caused by various Aeromonas.Mangrove sediments possess abundant Bacillus,becoming important reservoirs of bioactive microorganisms.In this study,we isolated two strains from mangrove sediments in Dongchong,Shenzhen,which displayed strong antagonistic activity against six pathogenic Aeromonas species.Based on the analysis of morphological properties,biochemical characteristics and molecular genetics,the two strains named AH10 and AQ1 were identified as B.amyloliquefaciens and B.velezensis respectively.The biological characteristics of the two strains showed similar optimum conditions of 32℃,pH of 5—7 and salinity of 0—20 for growth.The results of antibiotics sensitivity test showed that the two strains were highly sensitive to most of the antibiotics,except tetracycline and low dose streptomycin.Besides the six pathogenic Aeromonas,the antagonistic results revealed that the two strains also had antagonistic activities against Edwardsiella,and Plesiomonas shigelloides,in which B.amyloliquefaciens AH10 and B.velezensis AQ1 showed the strongest effect against P.shigelloides and A.salmonicida respectively.The two strains also showed no pathogenic capacity to zebrafish Danio rerio and silver carp Hypophthalmichthys molitrix.In conclusion,new antagonistic resources for the biological control of pathogenic Aeromonas in aquaculture is reported,providing good application prospects and research potential in the future.