文献类型：期刊文献

中文题名：低温与无水胁迫下凡纳滨对虾肌肉品质劣变标记蛋白识别

英文题名：Identification of Marker Proteins Indicating Potential Flesh Quality Deterioration of Litopenaeus vannamei Under Low Temperature Stress andWaterless Stress

作者：徐德峰[1,2];廖威龙[1];郑晓娴[1];叶盛权[1];茅林春[3];谢晶[4];邓尚贵[5]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]广东医科大学海洋医药研究院,广东湛江524023;[3]浙江大学生物系统工程与食品科学学院,浙江杭州310058;[4]上海海洋大学食品科学学院,上海201306;[5]浙江海洋大学食品与药学学院,浙江舟山316004

年份：2024

卷号：44

期号：2

起止页码：76

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：北大核心2023、CSTPCD、、CSCD_E2023_2024、北大核心、CSCD

基金：“十三五”国家重点研发计划重点专项(2019YFD0901601);广东省自然科学基金(2023A1515010852)。

语种：中文

中文关键词：虾;低温暴露;无水保活;品质劣变;蛋白组组学;蛋白标志物

外文关键词：Litopenaeus vannamei;low-temperature exposure;water-free life-preservation;quality deterioration;proteomics;protein markers

中文摘要：【目的】探明凡纳滨对虾(Litopenaeus vannamei)保活流通过程中低温和无水双重胁迫诱导肌肉品质劣变与差异表达蛋白之间的相关性,识别品质劣变的潜在标记蛋白。【方法】在模拟的海水环境中[溶解氧质量浓度为7.2 mg/L,温度控制在(12±1)℃]观察对虾在低温和无水胁迫下的生理反应,通过串联质谱标签蛋白质组学方法,分析环境胁迫导致的肝胰腺蛋白质组显著差异性变化,并将其与肌肉品质进行相关性分析。【结果】与正常对照组相比,低温组和低温+无水组分别鉴定了33和44个显著差异蛋白质(DEPs),这些蛋白质大多在溶酶体、糖酵解或糖异生、黏附等通路显著富集(P<0.05),同时胰蛋白酶-1、二肽基肽酶显著上调(P<0.05),而磷酸丙糖异构酶和醛脱氢酶显著下调(P<0.05)。此外,颜色和质构指标的变化与微管蛋白、凝溶胶蛋白、层黏连蛋白、内源性蛋白酶和代谢酶表达水平显著相关。【结论】本研究初次探明肝胰腺DEPs与肌肉品质间的内在联系,识别的典型DEPs可作为品质劣变的潜在标志蛋白,为监测无水运输期间凡纳滨对虾肌肉品质劣变提供分子靶标。

外文摘要：【Objective】The aim of this study is to investigate the correlation between muscle quality deterioration and differently expressed protein in hepatopancreas of Litopenaeus vannamei under low temperature(LT)stress and waterless duration(WD)stress during the anaerobic transportation,and to identify the potentially proteomic markers indicating the quality deterioration.【Method】The physiological responses of shrimp under LT stress and WD stress were observed in a simulated marine environment with a dissolved oxygen content of 7.2 mg/L and a temperature control of(12±1)℃.The significant difference of hepatopancreas proteome induced by the dual stress was analyzed by tandem mass spectrometry,and its correlation with muscle quality was analyzed accordingly.【Results】Compared with the control,33 and 44 differently expressed proteins were identified in LT and LT+WD groups,respectively,among which the most DEPs were significantly enriched in lysosomal,glycolytic or gluconeogenic,and adhesion pathways(P<0.05),while trypsin-1 and dipeptidyl peptidase underwent significant up-regulatied(P<0.05)and phosphoglycan isomerase and aldehyde dehydrogenase were significantly down-regulated(P<0.05).Moreover,the deterioration in colour and texture attributes was closely associated with the expression of tubulin,gelsolin,laminin,endogenous protease and metabolic enzymes.【Conclusion】This research found out that the intrinsic relationship between hepatopancreas DEPs and muscle quality for the first time.The identified typical DEPs could be used as potential marker proteins indicating quality deterioration,and thus providing a molecular target for monitoring muscle quality deterioration of L.vannamei during waterless live transport.